Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | stopped-flow kinetics show that MtKDC is allosterically activated by 2-oxoacids, also amino acids are potent activators | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) and various Escherichia coli strains | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyruvate | weak substrate inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
(indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | |
0.4 | - |
pyruvate | - |
Mycobacterium tuberculosis | |
0.83 | - |
2-Oxohexanoate | - |
Mycobacterium tuberculosis | |
1.17 | - |
phenylpyruvate | - |
Mycobacterium tuberculosis | |
1.21 | - |
2-oxopentanoate | - |
Mycobacterium tuberculosis | |
20.25 | - |
2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | |
26.11 | - |
benzoylformate | - |
Mycobacterium tuberculosis | |
138.3 | - |
2-oxobutyrate | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59780 | - |
calculated from the deduced amino acid sequence | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WG37 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WG37 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange chromatography and gel filtration | Mycobacterium tuberculosis |
Renatured (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | (indol-3-yl)acetaldehyde + CO2 | - |
? | |
(indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis H37Rv | (indol-3-yl)acetaldehyde + CO2 | - |
? | |
2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | 2-methylbutanal + CO2 | - |
? | |
2-oxobutyrate | - |
Mycobacterium tuberculosis | propionaldehyde + CO2 | - |
? | |
2-oxohexanoate | - |
Mycobacterium tuberculosis | n-pentanal + CO2 | - |
? | |
2-oxoisohexanoate | - |
Mycobacterium tuberculosis | ? + CO2 | - |
? | |
2-oxoisopentanoate | - |
Mycobacterium tuberculosis | 2-methylpropanal + CO2 | - |
? | |
2-oxopentanoate | - |
Mycobacterium tuberculosis | n-butanal + CO2 | - |
? | |
4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis | 4-hydroxyphenylacetaldehyde + CO2 | - |
? | |
4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis H37Rv | 4-hydroxyphenylacetaldehyde + CO2 | - |
? | |
benzoylformate | - |
Mycobacterium tuberculosis | ? + CO2 | - |
? | |
additional information | enzyme is found to convert a broad spectrum of branched-chain and aromatic alpha-keto acids. Determined the catalytic constants for the conversion of 11 aliphatic, aromatic, and branched-chain alpha-keto acids | Mycobacterium tuberculosis | ? | - |
? | |
additional information | enzyme is found to convert a broad spectrum of branched-chain and aromatic alpha-keto acids. Determined the catalytic constants for the conversion of 11 aliphatic, aromatic, and branched-chain alpha-keto acids | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
Phenylpyruvate | - |
Mycobacterium tuberculosis | Phenylacetaldehyde + CO2 | - |
? | |
Phenylpyruvate | - |
Mycobacterium tuberculosis H37Rv | Phenylacetaldehyde + CO2 | - |
? | |
pyruvate | - |
Mycobacterium tuberculosis | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Mycobacterium tuberculosis H37Rv | acetaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-keto acid decarboxylase | - |
Mycobacterium tuberculosis |
MtKDC | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.14 | - |
pyruvate | - |
Mycobacterium tuberculosis | |
2.98 | - |
(indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | |
3.89 | - |
4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis | |
5.98 | - |
2-oxobutyrate | - |
Mycobacterium tuberculosis | |
6.95 | - |
2-oxopentanoate | - |
Mycobacterium tuberculosis | |
7.06 | - |
2-Oxohexanoate | - |
Mycobacterium tuberculosis | |
7.5 | - |
phenylpyruvate | - |
Mycobacterium tuberculosis | |
18.57 | - |
benzoylformate | - |
Mycobacterium tuberculosis | |
19.75 | - |
2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | |
28.35 | - |
2-oxoisopentanoate | - |
Mycobacterium tuberculosis | |
31.5 | - |
2-Oxoisohexanoate | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
124 | - |
pyruvate | weak substrate inhibition is detected for pyruvate | Mycobacterium tuberculosis |