Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.127 | - |
2-oxo-3-phenylpropanoate | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
0.234 | - |
Indole-3-pyruvate | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
0.264 | - |
4-methyl-2-oxopentanoic acid | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
0.6 | - |
2-oxohexanoic acid | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
0.63 | - |
3-(4-hydroxyphenyl)-2-oxopropanoic acid | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
1.3 | - |
2-oxopentanoic acid | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
1.3 | - |
4-(methylsulfanyl)-2-oxobutanoic acid | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
5.02 | - |
3-methyl-2-oxobutanoic acid | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
7.5 | - |
oxo(phenyl)acetic acid | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis | |
29.77 | - |
2-Oxopropanoic acid | in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
2 * 73000, gel filtration | Lactococcus lactis |
126700 | - |
calculated from amino acid sequence | Lactococcus lactis |
146000 | - |
gel filtration | Lactococcus lactis |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | completely stable in the presence of 20% (v/v) DMSO (half-life: 150 h) | Lactococcus lactis |
polyethyleneglycol | rapidly inactivated in the presence of 15% (v/v) PEG-400 | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
subspecies Lactococcus lactis cremoris, strain B1157 | - |
Purification (Comment) | Organism |
---|---|
immobilized metal chelate chromatography and gel filtration | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-phenylpropanoic acid | at 30 mM 8.6% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | phenylacetaldehyde + CO2 | - |
? | |
2-oxobutanoic acid | at 30 mM 9.3% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | propanal + CO2 | - |
? | |
2-oxohexanoic acid | at 30 mM 13% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | pentanal + CO2 | - |
? | |
2-oxopentanoic acid | at 30 mM 15% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | butanal + CO2 | - |
? | |
2-oxopropanoic acid | at 30 mM 1.2% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | acetaldehyde + CO2 | - |
? | |
3-(4-hydroxyphenyl)-2-oxopropanoic acid | at 30 mM 6% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | (4-hydroxyphenyl)-acetaldehyde + CO2 | - |
? | |
3-methyl-2-oxobutanoic acid | 100% activity | Lactococcus lactis | 2-methylpropanal + CO2 | - |
? | |
3-methyl-2-oxopentanoic acid | at 30 mM 38.1% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | 2-methylbutanal + CO2 | - |
? | |
4-(4-hydroxyphenyl)-2-oxobutanoic acid | at 30 mM 1.6% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | 3-(4-hydroxyphenyl)-propanal + CO2 | - |
? | |
4-(methylsulfanyl)-2-oxobutanoic acid | at 30 mM 18% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | 3-(methylsulfanyl)-propanal + CO2 | - |
? | |
4-methyl-2-oxohexanoic acid | at 30 mM 19% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | 3-methylpentanal + CO2 | - |
? | |
4-methyl-2-oxopentanoic acid | at 30 mM 26.3% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | 3-methylbutanal + CO2 | - |
? | |
5-(4-hydroxyphenyl)-2-oxopentanoic acid | at 30 mM 1.1% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | 4-(4-hydroxyphenyl)-butanal + CO2 | - |
? | |
benzaldehyde + CO2 | self-carboligation | Lactococcus lactis | (R)-benzoin | - |
? | |
indole-3-pyruvate | at 1 mM 0.85% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | ? | - |
? | |
isovaleraldehyde | self-carboligation | Lactococcus lactis | ? | - |
? | |
additional information | 3-fluoro-2-oxopropanoic acid, isobutyraldehyde, 3,5-dichlorobenzaldehyde, 3,5-dimethoxybenzaldehyde and 2-oxooctanoic acid are no substrates | Lactococcus lactis | ? | - |
? | |
oxo(phenyl)acetic acid | at 30 mM 8.4% activity relative to 3-methyl-2-oxobutanoic acid | Lactococcus lactis | benzaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 73000, gel filtration | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
branched-chain keto acid decarboxylase | - |
Lactococcus lactis |
KdcA | has decarboxylase and carboligase activities | Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Lactococcus lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
sufficiently stable up to 40°C, but rapidly loses activity at higher temperatures | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Lactococcus lactis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
rapid inactivation at pH 4 | Lactococcus lactis |
5 | 7 | stable at pH 5-7 | Lactococcus lactis |
8 | - |
inactivation at pH 8 | Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent | Lactococcus lactis |