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Literature summary for 4.1.1.72 extracted from

  • Gocke, D.; Nguyen, C.L.; Pohl, M.; Stillger, T.; Walter, L.; Mueller, M.
    Branched-chain keto acid decarboxylase from Lactococcus lactis (KdcA), a valuable thiamine diphosphate-dependent enzyme for asymmetric C-C bond formation (2007), Adv. Synth. Catal., 349, 1425-1435.
No PubMed abstract available
Search for more literature for 4.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.127
-
 2-oxo-3-phenylpropanoate in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
0.234
-
 Indole-3-pyruvate in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
0.264
-
 4-methyl-2-oxopentanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
0.6
-
 2-oxohexanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
0.63
-
 3-(4-hydroxyphenyl)-2-oxopropanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
1.3
-
 2-oxopentanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
1.3
-
 4-(methylsulfanyl)-2-oxobutanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
5.02
-
 3-methyl-2-oxobutanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
7.5
-
 oxo(phenyl)acetic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
29.77
-
 2-Oxopropanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 2 * 73000, gel filtration Lactococcus lactis
126700
-
 calculated from amino acid sequence Lactococcus lactis
146000
-
 gel filtration Lactococcus lactis

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO completely stable in the presence of 20% (v/v) DMSO (half-life: 150 h) Lactococcus lactis
polyethyleneglycol rapidly inactivated in the presence of 15% (v/v) PEG-400 Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 subspecies Lactococcus lactis cremoris, strain B1157
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal chelate chromatography and gel filtration Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-phenylpropanoic acid at 30 mM 8.6% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis phenylacetaldehyde + CO2
-
 ?
2-oxobutanoic acid at 30 mM 9.3% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis propanal + CO2
-
 ?
2-oxohexanoic acid at 30 mM 13% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis pentanal + CO2
-
 ?
2-oxopentanoic acid at 30 mM 15% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis butanal + CO2
-
 ?
2-oxopropanoic acid at 30 mM 1.2% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis acetaldehyde + CO2
-
 ?
3-(4-hydroxyphenyl)-2-oxopropanoic acid at 30 mM 6% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis (4-hydroxyphenyl)-acetaldehyde + CO2
-
 ?
3-methyl-2-oxobutanoic acid 100% activity Lactococcus lactis 2-methylpropanal + CO2
-
 ?
3-methyl-2-oxopentanoic acid at 30 mM 38.1% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 2-methylbutanal + CO2
-
 ?
4-(4-hydroxyphenyl)-2-oxobutanoic acid at 30 mM 1.6% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 3-(4-hydroxyphenyl)-propanal + CO2
-
 ?
4-(methylsulfanyl)-2-oxobutanoic acid at 30 mM 18% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 3-(methylsulfanyl)-propanal + CO2
-
 ?
4-methyl-2-oxohexanoic acid at 30 mM 19% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 3-methylpentanal + CO2
-
 ?
4-methyl-2-oxopentanoic acid at 30 mM 26.3% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 3-methylbutanal + CO2
-
 ?
5-(4-hydroxyphenyl)-2-oxopentanoic acid at 30 mM 1.1% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 4-(4-hydroxyphenyl)-butanal + CO2
-
 ?
benzaldehyde + CO2 self-carboligation Lactococcus lactis (R)-benzoin
-
 ?
indole-3-pyruvate at 1 mM 0.85% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis ?
-
 ?
isovaleraldehyde self-carboligation Lactococcus lactis ?
-
 ?
additional information 3-fluoro-2-oxopropanoic acid, isobutyraldehyde, 3,5-dichlorobenzaldehyde, 3,5-dimethoxybenzaldehyde and 2-oxooctanoic acid are no substrates Lactococcus lactis ?
-
 ?
oxo(phenyl)acetic acid at 30 mM 8.4% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis benzaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 73000, gel filtration Lactococcus lactis

Synonyms

Synonyms Comment Organism
branched-chain keto acid decarboxylase
-
 Lactococcus lactis
KdcA has decarboxylase and carboligase activities Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Lactococcus lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 sufficiently stable up to 40°C, but rapidly loses activity at higher temperatures Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Lactococcus lactis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 rapid inactivation at pH 4 Lactococcus lactis
5 7 stable at pH 5-7 Lactococcus lactis
8
-
 inactivation at pH 8 Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent Lactococcus lactis