Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Synechococcus sp. PCC 7002 |
expression in Escherichia coli | Synechococcus sp. PCC 7002 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2 mM, 70% residual activity; about 60% residual activity at 2 mM Co2+ | Synechococcus sp. PCC 7002 | |
Cu2+ | 2 mM, 70% residual activity; about 50% residual activity at 2 mM Co2+ | Synechococcus sp. PCC 7002 | |
Zn2+ | 2 mM, no residual activity; the activity of the enzyme vanishes at 2 mM Zn2+ | Synechococcus sp. PCC 7002 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
2-oxoglutarate | pH 7.0, 25°C | Synechococcus sp. PCC 7002 | |
0.19 | - |
2-oxoglutarate | at pH 7.0 and 25°C | Synechococcus sp. PCC 7002 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, 1.8fold increase in activity | Synechococcus sp. PCC 7002 | |
Ca2+ | about 1.8fold increase of activity at 2 mM | Synechococcus sp. PCC 7002 | |
Mg2+ | 2 mM, 2.3fold increase in activity. Km value 0.059 mM | Synechococcus sp. PCC 7002 | |
Mg2+ | best stimulant, about 2.3fold increase of activity at 2 mM (used in assay conditions) | Synechococcus sp. PCC 7002 | |
Mn2+ | 2 mM, 2.2fold increase in activity | Synechococcus sp. PCC 7002 | |
Mn2+ | about 2.2fold increase of activity at 2 mM | Synechococcus sp. PCC 7002 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
247000 | - |
gel filtration | Synechococcus sp. PCC 7002 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate | Synechococcus sp. PCC 7002 | - |
succinate semialdehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. PCC 7002 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography, and Superdex 200 gel filtration | Synechococcus sp. PCC 7002 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate | - |
Synechococcus sp. PCC 7002 | succinate semialdehyde + CO2 | - |
? | |
additional information | no substrates: pyruvic acid, benzoylformic acid, 2-oxopentanoic acid, 4-methyl-2-oxopentanoic acid and 3-methyl-2-oxobutanoic acid. Enzyme additionally displays low acetolactate synthase activity | Synechococcus sp. PCC 7002 | ? | - |
? | |
additional information | the enzyme has pretty low activity of acetolactate synthase. The enzyme does not catalyze the decarboxylation of any of pyruvic acid, benzoylformic acid, 2-oxopentanoic acid, 4-methyl-2-oxopentanoic acid and 3-methyl-2-oxobutanoic acid to form corresponding aldehyde | Synechococcus sp. PCC 7002 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 60000, SDS-PAGE | Synechococcus sp. PCC 7002 |
tetramer | 4 * 60000, SDS-PAGE, * 61667, calcuated from sequence, recombinant protein with His-tag | Synechococcus sp. PCC 7002 |
Synonyms | Comment | Organism |
---|---|---|
alpha-ketoglutarate decarboxylase | - |
Synechococcus sp. PCC 7002 |
alpha-KGD | - |
Synechococcus sp. PCC 7002 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Synechococcus sp. PCC 7002 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | the enzyme activity drops by 50% at temperature lower than 40°C, is 70% at 55°C and almost lost at 60°C | Synechococcus sp. PCC 7002 |
40 | - |
less than 50% of maximum activity below | Synechococcus sp. PCC 7002 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
2-oxoglutarate | pH 7.0, 25°C | Synechococcus sp. PCC 7002 | |
1.2 | - |
2-oxoglutarate | at pH 7.0 and 25°C | Synechococcus sp. PCC 7002 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Synechococcus sp. PCC 7002 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
34% residual activity | Synechococcus sp. PCC 7002 |
6.5 | 7 | the optimal pH for the enzyme is at the range of 6.5-7.0. The activity of the enzyme is 34% and 17% at pH 6.0 and 8.0, respectively | Synechococcus sp. PCC 7002 |
8 | - |
17% residual activity | Synechococcus sp. PCC 7002 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | required for activity | Synechococcus sp. PCC 7002 | |
thiamine diphosphate | Km value 0.031 mM | Synechococcus sp. PCC 7002 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.3 | - |
2-oxoglutarate | pH 7.0, 25°C | Synechococcus sp. PCC 7002 | |
6.3 | - |
2-oxoglutarate | at pH 7.0 and 25°C | Synechococcus sp. PCC 7002 |