Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C15A/C17A | the mutant displays a decarboxylation activity similar to that of the wild type enzyme | Homo sapiens |
H169A/D171A | the mutant is devoid of the ability to perform 2-oxoglutarate turnover | Homo sapiens |
General Stability | Organism |
---|---|
the isolated proteins (ALKBH4 and mutants), even when kept in iced-cooled solutions (4°C), aggregate and then precipitate at concentrations higher than 0.5 mM. The process is accelerated greatly when an excess (e.g. 5fold) of dithionite is added to the solution when anaerobic Fe(II)-reconstitution procedures are carried out | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography, gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate | - |
Homo sapiens | succinate semialdehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALKBH4 | - |
Homo sapiens |
Fe(II)/2OG-dependent decarboxylase | - |
Homo sapiens |