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Literature summary for 4.1.1.7 extracted from

  • Peper, S.; Kara, S.; Long, W.S.; Liese, A.; Niemeyer, B.
    Immobilization and characterization of benzoylformate decarboxylase from Pseudomonas putida on spherical silica carrier (2011), Bioprocess Biosyst. Eng., 34, 671-680.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli SG13009 cells Pseudomonas putida

General Stability

General Stability Organism
the activity of the spherical silica-immobilized BFD A460I-F464I variant is determined to be 70% related to the activity of the free enzyme Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19.4
-
benzaldehyde free mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C Pseudomonas putida
51.9
-
benzaldehyde immobilized mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C Pseudomonas putida
515.9
-
acetaldehyde immobilized mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C Pseudomonas putida
775
-
acetaldehyde free mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P20906
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + acetaldehyde
-
Pseudomonas putida 2-hydroxypropiophenone
-
?
benzoylformate
-
Pseudomonas putida benzaldehyde + CO2
-
?

Subunits

Subunits Comment Organism
homotetramer
-
Pseudomonas putida

Synonyms

Synonyms Comment Organism
BFD
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
temperature optimum for immobilized variant A460I/F464I Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Pseudomonas putida