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Literature summary for 4.1.1.7 extracted from

  • Berheide, M.; Peper, S.; Kara, S.; Long, W.S.; Schenkel, S.; Pohl, M.; Niemeyer, B.; Liese, A.
    Influence of the hydrostatic pressure and pH on the asymmetric 2-hydroxyketone formation catalyzed by Pseudomonas putida benzoylformate decarboxylase and variants thereof (2010), Biotechnol. Bioeng., 106, 18-26.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain SG13009 Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
A460I for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 Pseudomonas putida
A460I/F464I for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 Pseudomonas putida
F464I for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + acetaldehyde
-
Pseudomonas putida (S)-2-hydroxypropiophenone
-
?
benzylformate
-
Pseudomonas putida benzaldehyde + CO2
-
?

Synonyms

Synonyms Comment Organism
BFD
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent on Pseudomonas putida