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Literature summary for 4.1.1.7 extracted from
- Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201 (2007), Biochim. Biophys. Acta, 1770, 1585-1592.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas stutzeri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
benzoylformate | recombinant, his-tagged enzyme at pH 6.0 and 35°C | Pseudomonas stutzeri |  |
| 0.69 | - |
benzoylformate | wild type enzyme at pH 6.0 and 35°C | Pseudomonas stutzeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
4 * 55000, gel filtration | Pseudomonas stutzeri |
| 220000 | - |
gel filtration | Pseudomonas stutzeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
- |
- |
| Pseudomonas stutzeri ST-201 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Phenyl Sepharose FF column chromatography and Q Sepharose FF column chromatography | Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoylformate | - |
Pseudomonas stutzeri | benzaldehyde + CO2 | - |
? | |
| benzoylformate | - |
Pseudomonas stutzeri ST-201 | benzaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 55000, gel filtration | Pseudomonas stutzeri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BFDC | - |
Pseudomonas stutzeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | - |
Pseudomonas stutzeri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 222 | - |
benzoylformate | wild type enzyme at pH 6.0 and 35°C | Pseudomonas stutzeri | |
| 284 | - |
benzoylformate | recombinant, his-tagged enzyme at pH 6.0 and 35°C | Pseudomonas stutzeri | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudomonas stutzeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | dependent | Pseudomonas stutzeri | |
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