Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, structure of the enzyme complexed with the inhibitor (R)-mandelate | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
H281A | 4.5fold increase in Km-value for benzoylformate, 3443fold decrease in turnover number for benzoylformate, 171fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme | Pseudomonas putida |
H70A | 5.2fold increase in Km-value for benzoylformate, 3443fold decrease in turnover number for benzoylformate, 40fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme | Pseudomonas putida |
S26A | 23.1fold increase in Km-value for benzoylformate, 54fold decrease in turnover number for benzoylformate, 100fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-mandelate | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
benzoylformate | pH 7.0, wild-type enzyme | Pseudomonas putida | |
1.68 | - |
benzoylformate | pH 7.0, mutant enzyme H281A | Pseudomonas putida | |
1.92 | - |
benzoylformate | pH 7.0, mutant enzyme H70A | Pseudomonas putida | |
8.62 | - |
benzoylformate | pH 7.0, mutant enzyme S26A | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P20906 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
benzoylformate | pH 7.0, mutant enzyme H70A | Pseudomonas putida | |
1.41 | - |
benzoylformate | pH 7.0, mutant enzyme H281A | Pseudomonas putida | |
4.45 | - |
benzoylformate | pH 7.0, mutant enzyme S26A | Pseudomonas putida | |
241 | - |
benzoylformate | pH 7.0, wild-type enzyme | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
(R)-mandelate | pH 6.5, wild-type enzyme | Pseudomonas putida | |
40 | - |
(R)-mandelate | pH 6.5, mutant enzyme H281A | Pseudomonas putida | |
40 | - |
(R)-mandelate | pH 6.5, mutant enzyme H70A | Pseudomonas putida | |
100 | - |
(R)-mandelate | pH 6.5, mutant enzyme S26A | Pseudomonas putida |