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Literature summary for 4.1.1.7 extracted from

  • Polovnikova, E.S.; McLeish, M.J.; Sergienko, E.A.; Burgner, J.T.; Anderson, N.L.; Bera, A.K.; Jordan, F.; Kenyon, G.L.; Hasson, M.S.
    Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylase (2003), Biochemistry, 42, 1820-1830.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, structure of the enzyme complexed with the inhibitor (R)-mandelate Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
H281A 4.5fold increase in Km-value for benzoylformate, 3443fold decrease in turnover number for benzoylformate, 171fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme Pseudomonas putida
H70A 5.2fold increase in Km-value for benzoylformate, 3443fold decrease in turnover number for benzoylformate, 40fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme Pseudomonas putida
S26A 23.1fold increase in Km-value for benzoylformate, 54fold decrease in turnover number for benzoylformate, 100fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
(R)-mandelate
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 benzoylformate pH 7.0, wild-type enzyme Pseudomonas putida
1.68
-
 benzoylformate pH 7.0, mutant enzyme H281A Pseudomonas putida
1.92
-
 benzoylformate pH 7.0, mutant enzyme H70A Pseudomonas putida
8.62
-
 benzoylformate pH 7.0, mutant enzyme S26A Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P20906
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 benzoylformate pH 7.0, mutant enzyme H70A Pseudomonas putida
1.41
-
 benzoylformate pH 7.0, mutant enzyme H281A Pseudomonas putida
4.45
-
 benzoylformate pH 7.0, mutant enzyme S26A Pseudomonas putida
241
-
 benzoylformate pH 7.0, wild-type enzyme Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
 (R)-mandelate pH 6.5, wild-type enzyme Pseudomonas putida
40
-
 (R)-mandelate pH 6.5, mutant enzyme H281A Pseudomonas putida
40
-
 (R)-mandelate pH 6.5, mutant enzyme H70A Pseudomonas putida
100
-
 (R)-mandelate pH 6.5, mutant enzyme S26A Pseudomonas putida