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Literature summary for 4.1.1.65 extracted from

  • Choi, J.Y.; Duraisingh, M.T.; Marti, M.; Ben Mamoun, C.; Voelker, D.R.
    From protease to decarboxylase the molecular metamorphosis of phosphatidylserine decarboxylase (2015), J. Biol. Chem., 290, 10972-10980 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Plasmodium knowlesi

Protein Variants

Protein Variants Comment Organism
D139A mutation prevents processing of the proenzyme Plasmodium knowlesi
D139N mutation prevents processing of the proenzyme Plasmodium knowlesi
G307A mutation eliminates processing Plasmodium knowlesi
G307P mutation eliminates processing Plasmodium knowlesi
H195A mutation produces significant levels of enzyme processing, although the level of the beta-subunit is reduced compared with wild-type Plasmodium knowlesi
H195A/H198A mutation prevents processing of the proenzyme Plasmodium knowlesi
H198A mutation prevents processing of the proenzyme Plasmodium knowlesi
S*308A mutation eliminates processing Plasmodium knowlesi
S*308T mutation eliminates processing Plasmodium knowlesi
S309A proenzyme is processed comparably to wild-type, modest reductions in catalytic activity Plasmodium knowlesi
S309T proenzyme is processed comparably to wild-type, modest reductions in catalytic activity Plasmodium knowlesi

Organism

Organism UniProt Comment Textmining
Plasmodium knowlesi B3L2V1
-
-
Plasmodium knowlesi H B3L2V1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
acylation the pyruvoyl moiety is detected at the N-termini of alpha-subunits Plasmodium knowlesi
proteolytic modification the post-translational processing is inhibited by the serine protease inhibitor, phenylmethylsulfonyl fluoride. Residues Asp139, His198, and Ser308 are all essential for endoproteolytic processing of PSD, which occurs in cis. Within the GS(S/T) motif found in all PSDs, the Gly307 residue is also essential, but the Ser/Thr309 is nonessential Plasmodium knowlesi

Synonyms

Synonyms Comment Organism
PKH_072580
-
Plasmodium knowlesi
PSD
-
Plasmodium knowlesi