Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.61 extracted from

  • Liu, J.; Zhang, X.; Zhou, S.; Tao, P.; Liu, J.
    Purification and characterization of a 4-hydroxybenzoate decarboxylase from Chlamydophila pneumoniae AR39 (2007), Curr. Microbiol., 54, 102-107.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Chlamydia pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
4-hydroxybenzoate in 20 mM Tris-HCl (pH 7.5) containing 1 mM Mg2+, at 30┬░C Chlamydia pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.44fold stimulation of activity Chlamydia pneumoniae
Fe2+ 1 mM, 2.52fold stimulation of activity Chlamydia pneumoniae
Mg2+ 1 mM, 2.99fold stimulation of activity Chlamydia pneumoniae
Mn2+ 1 mM, 2.43fold stimulation of activity Chlamydia pneumoniae
additional information not stimulated by Cu2+ or Zn2+ Chlamydia pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
SDS-PAGE Chlamydia pneumoniae

Organism

Organism UniProt Comment Textmining
Chlamydia pneumoniae
-
-
-
Chlamydia pneumoniae AR39
-
-
-

Purification (Commentary)

Purification (Comment) Organism
under anaerobic conditions (oxygen-free N2) at 4┬░C by affinity chromatography Chlamydia pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.00025
-
crude cell extract, at 30┬░C Chlamydia pneumoniae
0.00506
-
after purification, at 30┬░C Chlamydia pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Hydroxybenzoate
-
Chlamydia pneumoniae Phenol + CO2
-
r
4-Hydroxybenzoate
-
Chlamydia pneumoniae AR39 Phenol + CO2
-
r
Phenol + CO2 weak reaction Chlamydia pneumoniae 4-Hydroxybenzoate
-
r
Phenol + CO2 weak reaction Chlamydia pneumoniae AR39 4-Hydroxybenzoate
-
r

Synonyms

Synonyms Comment Organism
HBDC
-
Chlamydia pneumoniae

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Chlamydia pneumoniae

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40 50 loses more than 50% decarboxylase activity at 40┬░C for 5 min, and less than 25% enzyme activity remains after incubation at 50┬░C for 5 min Chlamydia pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Chlamydia pneumoniae