Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.61 extracted from

  • Matsui, T.; Yoshida, T.; Hayashi, T.; Nagasawa, T.
    Purification, characterization, and gene cloning of 4-hydroxybenzoate decarboxylase of Enterobacter cloacae P240 (2006), Arch. Microbiol., 186, 21-29.
    View publication on PubMed
Search for more literature for 4.1.1.61

Activating Compound

Activating Compound Comment Organism Structure
additional information 1 mM pyridoxal 5'-phosphate or thiamine diphosphate do not enhance activity Enterobacter cloacae

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 2.5 mM, 91% loss of activity Enterobacter cloacae
CuSO4 2.5 mM, 92% loss of activity Enterobacter cloacae
EDTA 2.5 mM, 22% inhibition Enterobacter cloacae
HgCl2 2.5 mM, 78% loss of activity Enterobacter cloacae
additional information not significantly affected by phenylhydrazine or hydroxylamine Enterobacter cloacae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.596
-
 4-hydroxybenzoate in 100 mM potassium phosphate buffer (pH 7.0) Enterobacter cloacae
6.8
-
 3,4-dihydroxybenzoate in 100 mM potassium phosphate buffer (pH 7.0) Enterobacter cloacae
14.8
-
 phenol 20 mM phenol, 3 MKHCO3, 10 mM dithiothreitol in 100 mM potassium phosphate buffer (pH 7.0) Enterobacter cloacae

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 2.5 mM, 99% stimulation of activity Enterobacter cloacae
CdCl2 2.5 mM, 51% stimulation of activity Enterobacter cloacae
MgSO4 2.5 mM, 125% stimulation of activity Enterobacter cloacae
MnCl2 2.5 mM, 112% stimulation of activity Enterobacter cloacae
additional information AgNO3, FeCl2, CoCl2, NiCl2, and FeSO4 do not affect activity Enterobacter cloacae
ZnCl2 2.5 mM, 75% stimulation of activity Enterobacter cloacae
ZnSO4 2.5 mM, 100% stimulation of activity Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 6 * 60000, X-ray crystallography Enterobacter cloacae
315800
-
 calculated from amino acid sequence Enterobacter cloacae
372000
-
 gel-filtration HPLC Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
-
-
Enterobacter cloacae P240
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel column chromatography, phenyl-Sepharose CL-4B column chromatography, and butyl-Toyopearl 650M column chromatography Enterobacter cloacae

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-hydroxybenzoate-grown cell
-
 Enterobacter cloacae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.7
-
 at 30°C, pH 7.0, crude cell extract Enterobacter cloacae
17.8
-
 at 30°C, pH 7.0, after 1.5fold purification Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate activity toward 3,4-dihydroxybenzoate at 5 mM is 2.8% of that toward 4-hydroxybenzoate Enterobacter cloacae benzene-1,2-diol + CO2
-
 ?
3,4-dihydroxybenzoate activity toward 3,4-dihydroxybenzoate at 5 mM is 2.8% of that toward 4-hydroxybenzoate Enterobacter cloacae P240 benzene-1,2-diol + CO2
-
 ?
4-Hydroxybenzoate
-
 Enterobacter cloacae Phenol + CO2
-
 r
4-Hydroxybenzoate
-
 Enterobacter cloacae P240 Phenol + CO2
-
 r
additional information benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, 2,6-dihydroxybenzoate, 2,4,6-trihydroxybenzoate, 3,4,5-trihydroxybenzoate, terephthalate, vanillate, 4-methoxybenzoate, 4-aminobenzoate, and 6-hydroxy-2-naphthoate are inert as substrates Enterobacter cloacae ?
-
 ?
additional information benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, 2,6-dihydroxybenzoate, 2,4,6-trihydroxybenzoate, 3,4,5-trihydroxybenzoate, terephthalate, vanillate, 4-methoxybenzoate, 4-aminobenzoate, and 6-hydroxy-2-naphthoate are inert as substrates Enterobacter cloacae P240 ?
-
 ?
Phenol + CO2 carboxylation in the presence of 3 M KHCO3 and 20 mM phenol with a molar conversion yield of 19% Enterobacter cloacae 4-Hydroxybenzoate
-
 ?
Phenol + CO2 carboxylation in the presence of 3 M KHCO3 and 20 mM phenol with a molar conversion yield of 19% Enterobacter cloacae P240 4-Hydroxybenzoate
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 60000, X-ray crystallography Enterobacter cloacae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in potassium phosphate buffer Enterobacter cloacae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 when the enzyme is incubated at temperatures up to 20°C for 30 min, activity is barely decreased Enterobacter cloacae
30 60 treatment at 30, 40, 50, and 60°C causes 32, 43, 96, and 100% losses of the initial activity, respectively Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 in potassium phosphate buffer Enterobacter cloacae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5
-
 Enterobacter cloacae

Cofactor

Cofactor Comment Organism Structure
additional information the activity of 4-hydroxybenzoate decarboxylase is independent of pyridoxal 5'-phosphate, thiamine diphosphate, and the pyruvoyl group Enterobacter cloacae