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Literature summary for 4.1.1.61 extracted from

  • Li, T.; Juteau, P.; Beaudet, R.; Lepine, F.; Villemur, R.; Bisaillon, J.G.
    Purification and characterization of a 4-hydroxybenzoate decarboxylase from an anaerobic coculture (2000), Can. J. Microbiol., 46, 856-859.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no stimulation by ATP up to 5 mM or phenylphosphate uncultured bacterium

General Stability

General Stability Organism
oxygen sensitive, loss of 50% of activity after 1 h in presence of air at 4┬░C compared to no activity lost after 7 days under anaerobic conditions at 4┬░C uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
EDTA 47% inhibition at 5 mM, Mg2+, Mn2+, Zn2+ or Ca2+ protect, Fe2+ does not uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4
-
4-hydroxybenzoate pH 6.0, 37┬░C uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
Clostridium-like strain 6
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
766
-
-
uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate slow reaction uncultured bacterium benzene-1,2-diol + CO2
-
?
4-Hydroxybenzoate
-
uncultured bacterium Phenol + CO2
-
?
additional information no substrates are 2-hydroxybenzoate, 3-hydroxybenzoate, 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 2,3,4-trihydroxybenzoate, 3,4,5-trihydroxybenzoate uncultured bacterium ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.5 broad uncultured bacterium

pI Value

Organism Comment pI Value Maximum pI Value
uncultured bacterium isoeolctric focusing 5.9 5.6