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Literature summary for 4.1.1.61 extracted from

  • He, Z.; Wiegel, J.
    Purification and characterization of an oxygen-sensitive reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum (1995), Eur. J. Biochem., 229, 77-82.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,3,4-Trihydroxybenzoate
-
Sedimentibacter hydroxybenzoicus
2,4,6-Trihydroxybenzoate
-
Sedimentibacter hydroxybenzoicus
2,4-Dihydroxybenzoate
-
Sedimentibacter hydroxybenzoicus
3,4,5-Trihydroxybenzoate
-
Sedimentibacter hydroxybenzoicus
NH4+ decreases carboxylation of 3,4-dihydroxybenzoate by about 20% Sedimentibacter hydroxybenzoicus
Rb+ decreases carboxylation of 3,4-dihydroxybenzoate by about 20% Sedimentibacter hydroxybenzoicus
Zn2+ destroys carboxylation activity towards 4-hydroxybenzoate and 3,4-dihydroxybenzoate Sedimentibacter hydroxybenzoicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
4-hydroxybenzoate
-
Sedimentibacter hydroxybenzoicus
1.2
-
3,4-dihydroxybenzoate
-
Sedimentibacter hydroxybenzoicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ minor stimulation of carboxylation of 4-hydroxybenzoate Sedimentibacter hydroxybenzoicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
6 * 57000, SDS-PAGE Sedimentibacter hydroxybenzoicus
350000
-
gradient PAGE, gel filtration Sedimentibacter hydroxybenzoicus

Organism

Organism UniProt Comment Textmining
Sedimentibacter hydroxybenzoicus
-
Clostridium hydroxybenzoicum, strain JW/Z-1T
-

Oxidation Stability

Oxidation Stability Organism
oxygen-sensitive, 50% loss of activity when exposed to air at 5┬░C for 2 h Sedimentibacter hydroxybenzoicus

Purification (Commentary)

Purification (Comment) Organism
-
Sedimentibacter hydroxybenzoicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
8.29
-
-
Sedimentibacter hydroxybenzoicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-Dihydroxybenzoate
-
Sedimentibacter hydroxybenzoicus Catechol + CO2
-
?
4-Hydroxybenzoate
-
Sedimentibacter hydroxybenzoicus Phenol + CO2
-
?
Catechol + CO2
-
Sedimentibacter hydroxybenzoicus 3,4-Dihydroxybenzoate
-
?
Phenol + CO2
-
Sedimentibacter hydroxybenzoicus 4-Hydroxybenzoate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 57000, SDS-PAGE Sedimentibacter hydroxybenzoicus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
50
-
-
Sedimentibacter hydroxybenzoicus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
11 40 pH 6.0, stable for more than 24 h Sedimentibacter hydroxybenzoicus
50
-
half-life: 12 h Sedimentibacter hydroxybenzoicus
56
-
half-life: 15 min Sedimentibacter hydroxybenzoicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.3
-
3,4-dihydroxybenzoate
-
Sedimentibacter hydroxybenzoicus
55
-
4-hydroxybenzoate
-
Sedimentibacter hydroxybenzoicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 6.2
-
Sedimentibacter hydroxybenzoicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.3 stable for 2 h Sedimentibacter hydroxybenzoicus
6
-
11-40┬░C, stable for more than 24 h Sedimentibacter hydroxybenzoicus