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Literature summary for 4.1.1.61 extracted from
- Purification and characterization of an oxygen-sensitive reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum (1995), Eur. J. Biochem., 229, 77-82.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3,4-Trihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus |  |
| 2,4,6-Trihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
| 2,4-Dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
| 3,4,5-Trihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
| NH4+ | decreases carboxylation of 3,4-dihydroxybenzoate by about 20% | Sedimentibacter hydroxybenzoicus | |
| Rb+ | decreases carboxylation of 3,4-dihydroxybenzoate by about 20% | Sedimentibacter hydroxybenzoicus | |
| Zn2+ | destroys carboxylation activity towards 4-hydroxybenzoate and 3,4-dihydroxybenzoate | Sedimentibacter hydroxybenzoicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
4-hydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
| 1.2 | - |
3,4-dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | minor stimulation of carboxylation of 4-hydroxybenzoate | Sedimentibacter hydroxybenzoicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
6 * 57000, SDS-PAGE | Sedimentibacter hydroxybenzoicus |
| 350000 | - |
gradient PAGE, gel filtration | Sedimentibacter hydroxybenzoicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sedimentibacter hydroxybenzoicus | - |
Clostridium hydroxybenzoicum, strain JW/Z-1T | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen-sensitive, 50% loss of activity when exposed to air at 5°C for 2 h | Sedimentibacter hydroxybenzoicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sedimentibacter hydroxybenzoicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.29 | - |
- |
Sedimentibacter hydroxybenzoicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-Dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | Catechol + CO2 | - |
? | |
| 4-Hydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | Phenol + CO2 | - |
? | |
| Catechol + CO2 | - |
Sedimentibacter hydroxybenzoicus | 3,4-Dihydroxybenzoate | - |
? | |
| Phenol + CO2 | - |
Sedimentibacter hydroxybenzoicus | 4-Hydroxybenzoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 57000, SDS-PAGE | Sedimentibacter hydroxybenzoicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Sedimentibacter hydroxybenzoicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 11 | 40 | pH 6.0, stable for more than 24 h | Sedimentibacter hydroxybenzoicus |
| 50 | - |
half-life: 12 h | Sedimentibacter hydroxybenzoicus |
| 56 | - |
half-life: 15 min | Sedimentibacter hydroxybenzoicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.3 | - |
3,4-dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
| 55 | - |
4-hydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 6.2 | - |
Sedimentibacter hydroxybenzoicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.3 | stable for 2 h | Sedimentibacter hydroxybenzoicus |
| 6 | - |
11-40°C, stable for more than 24 h | Sedimentibacter hydroxybenzoicus |
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