Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.6 extracted from

  • Steiger, M.G.; Blumhoff, M.L.; Mattanovich, D.; Sauer, M.
    Biochemistry of microbial itaconic acid production (2013), Front. Microbiol., 4, 23.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
gene cadA, heterologous functional expression in citric acid producing Aspergillus niger under various constitutive promoters of different expression strength, heterologous expression of the gene in Saccharomyces cerevisiae Aspergillus terreus

Protein Variants

Protein Variants Comment Organism
additional information a truncated version of the Aspergillus niger pfkA gene exhibits a higher citric acid yield due to a reduced inhibition by citrate and ATP, the truncated pfkA version has also a positive impact on the itaconic acid accumulation when expressed in Aspergillus terreus Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.45
-
 cis-aconitate pH 6.2, 37°C Aspergillus terreus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Aspergillus terreus 5829
-
cytosol
-
 Aspergillus itaconicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, recombinant enzyme, SDS-PAGE Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-aconitate Aspergillus terreus
-
 itaconate + CO2
-
 ?
cis-aconitate Aspergillus itaconicus
-
 itaconate + CO2
-
 ?
cis-aconitate Aspergillus terreus NRRL1960
-
 itaconate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus itaconicus
-
 gene cadA
-
Aspergillus terreus
-
 gene cadA
-
Aspergillus terreus NRRL1960
-
 gene cadA
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information production of itaconic acid is achieved by the fermentation with Aspergillus terreus on sugar-containing media Aspergillus terreus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-aconitate
-
 Aspergillus terreus itaconate + CO2
-
 ?
cis-aconitate
-
 Aspergillus itaconicus itaconate + CO2
-
 ?
cis-aconitate
-
 Aspergillus terreus NRRL1960 itaconate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, recombinant enzyme, SDS-PAGE Aspergillus terreus

Synonyms

Synonyms Comment Organism
CadA
-
 Aspergillus terreus
CadA
-
 Aspergillus itaconicus
cis-aconitate decarboxylase
-
 Aspergillus terreus
cis-aconitate decarboxylase
-
 Aspergillus itaconicus
cis-Aconitic acid decarboxylase
-
 Aspergillus terreus
cis-Aconitic acid decarboxylase
-
 Aspergillus itaconicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Aspergillus terreus

General Information

General Information Comment Organism
evolution the enzyme belongs to the MmgE/PrpD family Aspergillus terreus
metabolism in the first steps of the citric acid cycle, citrate and cis-aconitate are formed, the enzymes preceding in the pathway, namely citrate synthase and aconitase, are found in the mitochondria. In the last step, the only itaconic acid pathway dedicated step, cis-aconitate decarboxylase (CadA) forms itaconic acid releasing carbon dioxide in the cytosol Aspergillus terreus
metabolism in the first steps of the citric acid cycle, citrate and cis-aconitate are formed, the enzymes preceding in the pathway, namely citrate synthase and aconitase, are found in the mitochondria. In the last step, the only itaconic acid pathway dedicated step, cis-aconitate decarboxylase (CadA) forms itaconic acid releasing carbon dioxide in the cytosol Aspergillus itaconicus