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Literature summary for 4.1.1.59 extracted from
- Purification and characterization of gallic acid decarboxylase from Pantoea agglomerans T71 (1998), Appl. Environ. Microbiol., 64, 4743-4747.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | synthesis of pyrogallol from readily available tannic acid by using the combined action of gallate decarboxylase and tannase in a two-enzyme resting cell bioconversion | Pantoea agglomerans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2S2O8 | - |
Pantoea agglomerans |  |
| 1,10-phenanthroline | - |
Pantoea agglomerans | |
| 2,2'-bipyridyl | - |
Pantoea agglomerans | |
| Ag2SO4 | - |
Pantoea agglomerans | |
| CuCl2 | - |
Pantoea agglomerans | |
| Cuprizone | - |
Pantoea agglomerans | |
| EDTA | - |
Pantoea agglomerans | |
| FeCl2 | slight | Pantoea agglomerans | |
| FeCl3 | slight | Pantoea agglomerans | |
| H2O2 | - |
Pantoea agglomerans | |
| HgCl2 | - |
Pantoea agglomerans | |
| K2CrO4 | - |
Pantoea agglomerans | |
| KCN | - |
Pantoea agglomerans | |
| NaNO3 | - |
Pantoea agglomerans | |
| p-chloromercuribenzoate | - |
Pantoea agglomerans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.96 | - |
3,4,5-Trihydroxybenzoate | - |
Pantoea agglomerans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | required as cofactor, contains 0.8 mol of iron per mol of enzyme subunit | Pantoea agglomerans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
6 * 57000, SDS-PAGE | Pantoea agglomerans |
| 320000 | - |
gel filtration | Pantoea agglomerans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pantoea agglomerans | - |
i.e. Enterobacter agglomerans, strain T71 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen-sensitive enzyme | Pantoea agglomerans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pantoea agglomerans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 150 | - |
- |
Pantoea agglomerans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM potassium phosphate buffer, pH 6.0, containing glycerol and reducing agents, stable for 3 weeks | Pantoea agglomerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4,5-Trihydroxybenzoate | reverse reaction not catalyzed | Pantoea agglomerans | Pyrogallol + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 57000, SDS-PAGE | Pantoea agglomerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pantoea agglomerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable below | Pantoea agglomerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pantoea agglomerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | stable in the range | Pantoea agglomerans |
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