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Literature summary for 4.1.1.57 extracted from

  • Hayashi, M.; Okada, A.; Yamamoto, K.; Okugochi, T.; Kusaka, C.; Kudou, D.; Nemoto, M.; Inagaki, J.; Hirose, Y.; Okajima, T.; Tamura, T.; Soda, K.; Inagaki, K.
    Gene cloning, recombinant expression, purification and characterization of L-methionine decarboxylase from Streptomyces sp. 590 (2017), J. Biochem., 161, 389-398 .
    View publication on PubMed
Search for more literature for 4.1.1.57

Application

Application Comment Organism
medicine recombinant MetDC (0.50 U/ml) severely inhibits growth of human tumour cells A431 and MDA-MB-231, but shows relatively low cytotoxicity for human normal cell NHDF-Neo Streptomyces sp. 590 KI-2014

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp. 590 KI-2014

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
30
-
 L-methionine pH 6.6, 55°C Streptomyces sp. 590 KI-2014
73
-
 L-norleucine pH 6.6, 55°C Streptomyces sp. 590 KI-2014

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel filtration Streptomyces sp. 590 KI-2014

Organism

Organism UniProt Comment Textmining
Streptomyces sp. 590 KI-2014 A0A0G4DBU7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9
-
 pH 6.6, 55°C Streptomyces sp. 590 KI-2014

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine
-
 Streptomyces sp. 590 KI-2014 3-(methylsulfanyl)propanamine + CO2
-
 ?
L-norleucine
-
 Streptomyces sp. 590 KI-2014 pentanamine + CO2
-
 ?
S-methyl-L-cysteine
-
 Streptomyces sp. 590 KI-2014 2-methylthioethanamine + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 61000, SDS-PAGE Streptomyces sp. 590 KI-2014

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 55
-
 Streptomyces sp. 590 KI-2014

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable below Streptomyces sp. 590 KI-2014

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
61
-
 L-norleucine pH 6.6, 55°C Streptomyces sp. 590 KI-2014
239
-
 L-methionine pH 6.6, 55°C Streptomyces sp. 590 KI-2014

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
 Streptomyces sp. 590 KI-2014

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.6 7.6
-
 Streptomyces sp. 590 KI-2014

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Streptomyces sp. 590 KI-2014

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.83
-
 L-norleucine pH 6.6, 55°C Streptomyces sp. 590 KI-2014
8
-
 L-methionine pH 6.6, 55°C Streptomyces sp. 590 KI-2014