General Stability | Organism |
---|---|
little effect for stabilization to add pyridoxal 5'-phosphate, glycerol or 2-mercaptoethanol | Crypthecodinium cohnii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carboxymethoxylamine hemihydrochloride | 1 mM, 38.9% activity | Crypthecodinium cohnii | |
hydroxylamine | 1 mM, 1.6% activity | Crypthecodinium cohnii | |
phenylhydrazine | 1 mM, 29% activity | Crypthecodinium cohnii | |
Semicarbazide hydrochloride | 1 mM, 21.6% activity | Crypthecodinium cohnii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme is not membrane bound, because its activity can be solubilized into the 50 mM potassium phosphate buffer pH 7.2 without detergents | Crypthecodinium cohnii | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
2 * 100000, SDS-PAGE | Crypthecodinium cohnii |
204000 | - |
gel filtration chromatography | Crypthecodinium cohnii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine | Crypthecodinium cohnii | - |
3-methylthiopropanamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crypthecodinium cohnii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
four purification steps including anion-exchange chromatography and size-exclusion chromatography | Crypthecodinium cohnii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
crude extract | Crypthecodinium cohnii |
4.45 | - |
after sequential DEAE-Cellulose, Resource-Q, Mono-Q and Superdex 200 chromatography | Crypthecodinium cohnii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine | - |
Crypthecodinium cohnii | 3-methylthiopropanamine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 100000, SDS-PAGE | Crypthecodinium cohnii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Crypthecodinium cohnii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | retains approximately 70% activity in this range | Crypthecodinium cohnii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Crypthecodinium cohnii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | potassium phosphate buffer, values between 66% and 85% activity with respect to optimum pH | Crypthecodinium cohnii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 0.1 mM: 100% activity, 0.001 mM: 50.4% activity, 0.00001 mM: 30.4% activity. No pyridoxal 5'-phosphate added: 22% activity | Crypthecodinium cohnii |