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Literature summary for 4.1.1.57 extracted from

  • Kitaguchi, H.; Uchida, A.; Ishida, Y.
    Purification and characterization of L-methionine decarboxylase from Crypthecodinium cohnii (1999), Fish. Sci., 65, 613-617.
No PubMed abstract available

General Stability

General Stability Organism
little effect for stabilization to add pyridoxal 5'-phosphate, glycerol or 2-mercaptoethanol Crypthecodinium cohnii

Inhibitors

Inhibitors Comment Organism Structure
carboxymethoxylamine hemihydrochloride 1 mM, 38.9% activity Crypthecodinium cohnii
hydroxylamine 1 mM, 1.6% activity Crypthecodinium cohnii
phenylhydrazine 1 mM, 29% activity Crypthecodinium cohnii
Semicarbazide hydrochloride 1 mM, 21.6% activity Crypthecodinium cohnii

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme is not membrane bound, because its activity can be solubilized into the 50 mM potassium phosphate buffer pH 7.2 without detergents Crypthecodinium cohnii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
2 * 100000, SDS-PAGE Crypthecodinium cohnii
204000
-
gel filtration chromatography Crypthecodinium cohnii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine Crypthecodinium cohnii
-
3-methylthiopropanamine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Crypthecodinium cohnii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
four purification steps including anion-exchange chromatography and size-exclusion chromatography Crypthecodinium cohnii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.021
-
crude extract Crypthecodinium cohnii
4.45
-
after sequential DEAE-Cellulose, Resource-Q, Mono-Q and Superdex 200 chromatography Crypthecodinium cohnii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine
-
Crypthecodinium cohnii 3-methylthiopropanamine + CO2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 100000, SDS-PAGE Crypthecodinium cohnii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Crypthecodinium cohnii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 retains approximately 70% activity in this range Crypthecodinium cohnii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Crypthecodinium cohnii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 potassium phosphate buffer, values between 66% and 85% activity with respect to optimum pH Crypthecodinium cohnii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 0.1 mM: 100% activity, 0.001 mM: 50.4% activity, 0.00001 mM: 30.4% activity. No pyridoxal 5'-phosphate added: 22% activity Crypthecodinium cohnii