BRENDA - Enzyme Database
show all sequences of 4.1.1.53

Bacterial synthesis of N-hydroxycinnamoyl phenethylamines and tyramines

Sim, G.; Yang, S.; Kim, B.; Ahn, J.; Microb. Cell Fact. 14, 162 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene PDC, recombinant expression in Escherichia coli strain HP-2, coexpression with genes TDC, TAL, SHT, and THT
Pseudomonas putida
Engineering
Amino acid exchange
Commentary
Organism
additional information
genes PDC, TDC, TAL, SHT, and THT are introduced into engineered Escherichia coli strain HP-2 to synthesize N-(p-coumaroyl) phenethylamine and N-(p-coumaroyl) tyramine, method evaluation and optimization, overview. p-Coumaric acid is synthesized from tyrosine by TAL and phenethylamine is synthesized from phenylalanine by PDC. Therefore, the intracellular concentrations of both tyrosine and phenylalanine are critical for the final yield of N-(p-coumaroyl) phenethylamine
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-phenylalanine
Pseudomonas putida
-
phenylethylamine + CO2
-
-
?
L-phenylalanine
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
-
phenylethylamine + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
Q88JU5
-
-
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
Q88JU5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-phenylalanine
-
748581
Pseudomonas putida
phenylethylamine + CO2
-
-
-
?
L-phenylalanine
-
748581
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
phenylethylamine + CO2
-
-
-
?
additional information
enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine
748581
Pseudomonas putida
?
-
-
-
-
additional information
enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine
748581
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PDC, recombinant expression in Escherichia coli strain HP-2, coexpression with genes TDC, TAL, SHT, and THT
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Pseudomonas putida
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
genes PDC, TDC, TAL, SHT, and THT are introduced into engineered Escherichia coli strain HP-2 to synthesize N-(p-coumaroyl) phenethylamine and N-(p-coumaroyl) tyramine, method evaluation and optimization, overview. p-Coumaric acid is synthesized from tyrosine by TAL and phenethylamine is synthesized from phenylalanine by PDC. Therefore, the intracellular concentrations of both tyrosine and phenylalanine are critical for the final yield of N-(p-coumaroyl) phenethylamine
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-phenylalanine
Pseudomonas putida
-
phenylethylamine + CO2
-
-
?
L-phenylalanine
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
-
phenylethylamine + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-phenylalanine
-
748581
Pseudomonas putida
phenylethylamine + CO2
-
-
-
?
L-phenylalanine
-
748581
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
phenylethylamine + CO2
-
-
-
?
additional information
enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine
748581
Pseudomonas putida
?
-
-
-
-
additional information
enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine
748581
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
?
-
-
-
-
Other publictions for EC 4.1.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746609
Sytykiewicz
-
Methyl jasmonate elicitation ...
Zea mays subsp. mays
Acta Biol. Crac. Ser. Bot.
58
67-80
2016
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
-
-
-
1
1
2
2
-
-
748581
Sim
Bacterial synthesis of N-hydr ...
Pseudomonas putida, Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
Microb. Cell Fact.
14
162
2015
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728786
Stevenson
Expression of the DisA amino a ...
Proteus mirabilis
Res. Microbiol.
164
31-37
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
679891
Marcobal
First genetic characterization ...
Enterococcus faecium
FEMS Microbiol. Lett.
258
144-149
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654188
Ziadeh
Enzymatic decarboxylation of t ...
Enterococcus faecalis
Anal. Chem.
74
479-483
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4707
Buckberry
-
Cysteine conjugate beta-lyase ...
Enterococcus faecalis
Biochem. Soc. Trans.
26
269
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4231
Jebai
Expression, purification, and ...
Rattus norvegicus
Protein Expr. Purif.
11
185-194
1997
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
3
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
3
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4709
Taylor
-
Metabolism of D,L-chlorophenyl ...
no activity in Papaver somniferum, Phaseolus vulgaris
Biochemistry
26
1279-1288
1987
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4706
Chen
A rapid procedure for detectio ...
Morganella morganii
Anal. Biochem.
111
60-66
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
4708
Nakazawa
-
Distribution and formation of ...
Achromobacter sp., Micrococcus percitreus, Micrococcus sp., Sarcina sp., Staphylococcus sp.
Agric. Biol. Chem.
41
2241-2247
1977
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
-
-
-
1
-
21
-
5
-
-
-
5
-
-
5
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
21
-
5
-
-
-
5
-
-
-
-
-
-
-
-
-
4705
David
On the importance of decarboxy ...
Homo sapiens, Rattus norvegicus
Arch. Biochem. Biophys.
160
561-568
1974
-
1
-
-
-
-
2
-
-
-
-
3
-
2
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4704
Lovenberg
Aromatic L-amino acid decarbox ...
Canis lupus, Cavia aperea
J. Biol. Chem.
237
89-93
1962
1
-
-
-
-
-
6
6
-
-
-
1
-
2
-
-
2
-
-
6
-
1
13
-
1
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
6
-
6
-
-
-
1
-
-
-
2
-
6
-
1
13
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-