BRENDA - Enzyme Database
show all sequences of 4.1.1.53

Expression of the DisA amino acid decarboxylase from Proteus mirabilis inhibits motility and class 2 flagellar gene expression in Escherichia coli

Stevenson, L.G.; Szostek, B.A.; Clemmer, K.M.; Rather, P.N.; Res. Microbiol. 164, 31-37 (2013)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Proteus mirabilis
-
-
-
General Information
General Information
Commentary
Organism
physiological function
enzyme expression in Escherichia coli blocks motility and results in a 50fold decrease in the expression of class 2 and class 3 flagellar genes fliA and fliC, respectively. The expression of flhDC encoding the class 1 activator of the flagellarcascade is unchanged by the expression at both the transcriptional and translational levels. Phenethylamine, a decarboxylation product derived from phenylalanine, is able to mimic the overexpression and decrease both motility and class 2/3 flagellar gene expression. Both enzyme overexpression and phenethylamine strongly inhibit biofilm formation in Escherichia coli. Enzyme overexpression and exogenous phenethylamine can also reduce motility in other enteric bacteria, but have no effect on motility in non-enteric Gram-negative bacteria
Proteus mirabilis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme expression in Escherichia coli blocks motility and results in a 50fold decrease in the expression of class 2 and class 3 flagellar genes fliA and fliC, respectively. The expression of flhDC encoding the class 1 activator of the flagellarcascade is unchanged by the expression at both the transcriptional and translational levels. Phenethylamine, a decarboxylation product derived from phenylalanine, is able to mimic the overexpression and decrease both motility and class 2/3 flagellar gene expression. Both enzyme overexpression and phenethylamine strongly inhibit biofilm formation in Escherichia coli. Enzyme overexpression and exogenous phenethylamine can also reduce motility in other enteric bacteria, but have no effect on motility in non-enteric Gram-negative bacteria
Proteus mirabilis
Other publictions for EC 4.1.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746609
Sytykiewicz
-
Methyl jasmonate elicitation ...
Zea mays subsp. mays
Acta Biol. Crac. Ser. Bot.
58
67-80
2016
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
-
-
-
1
1
2
2
-
-
748581
Sim
Bacterial synthesis of N-hydr ...
Pseudomonas putida, Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
Microb. Cell Fact.
14
162
2015
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728786
Stevenson
Expression of the DisA amino a ...
Proteus mirabilis
Res. Microbiol.
164
31-37
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
679891
Marcobal
First genetic characterization ...
Enterococcus faecium
FEMS Microbiol. Lett.
258
144-149
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654188
Ziadeh
Enzymatic decarboxylation of t ...
Enterococcus faecalis
Anal. Chem.
74
479-483
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4707
Buckberry
-
Cysteine conjugate beta-lyase ...
Enterococcus faecalis
Biochem. Soc. Trans.
26
269
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4231
Jebai
Expression, purification, and ...
Rattus norvegicus
Protein Expr. Purif.
11
185-194
1997
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
3
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
3
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4709
Taylor
-
Metabolism of D,L-chlorophenyl ...
no activity in Papaver somniferum, Phaseolus vulgaris
Biochemistry
26
1279-1288
1987
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4706
Chen
A rapid procedure for detectio ...
Morganella morganii
Anal. Biochem.
111
60-66
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
4708
Nakazawa
-
Distribution and formation of ...
Achromobacter sp., Micrococcus percitreus, Micrococcus sp., Sarcina sp., Staphylococcus sp.
Agric. Biol. Chem.
41
2241-2247
1977
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
-
-
-
1
-
21
-
5
-
-
-
5
-
-
5
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
21
-
5
-
-
-
5
-
-
-
-
-
-
-
-
-
4705
David
On the importance of decarboxy ...
Homo sapiens, Rattus norvegicus
Arch. Biochem. Biophys.
160
561-568
1974
-
1
-
-
-
-
2
-
-
-
-
3
-
2
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4704
Lovenberg
Aromatic L-amino acid decarbox ...
Canis lupus, Cavia aperea
J. Biol. Chem.
237
89-93
1962
1
-
-
-
-
-
6
6
-
-
-
1
-
2
-
-
2
-
-
6
-
1
13
-
1
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
6
-
6
-
-
-
1
-
-
-
2
-
6
-
1
13
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-