Literature summary for 4.1.1.50 extracted from

Bale, S.; Baba, K.; McCloskey, D.E.; Pegg, A.E.; Ealick, S.E.
Complexes of Thermotoga maritima S-adenosylmethionine decarboxylase provide insights into substrate specificity (2010), Acta Crystallogr. Sect. D, 66, 181-189.

Search for more literature for 4.1.1.50

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli B834(DE3) cells	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complexes with S-adenosylmethionine methyl ester and 5'-deoxy-5'-dimethylthioadenosine, hanging drop vapor diffusion method, using 2.4-2.8 M ammonium formate and 100 mM HEPES pH 8.0	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	S-adenosyl-L-methionine	at pH 6.8 and 65°C	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9WZC3	proenzyme	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, gel filtration	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine	-	Thermotoga maritima	(5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2	-	?

Synonyms

Synonyms	Comment	Organism
AdoMetDC	-	Thermotoga maritima
S-Adenosylmethionine decarboxylase	-	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1	-	S-adenosyl-L-methionine	at pH 6.8 and 65°C	Thermotoga maritima

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Thermotoga maritima

General Information

General Information	Comment	Organism
metabolism	S-adenosylmethionine decarboxylase is a critical pyruvoyl-dependent enzyme in the polyamine-biosynthetic pathway	Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
12.5	-	S-adenosyl-L-methionine	at pH 6.8 and 65°C	Thermotoga maritima

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Release 2023.1 (January 2023)