Activating Compound | Comment | Organism | Structure |
---|---|---|---|
putrescine | enhances activity up to 350fold | Caenorhabditis elegans | |
spermidine | 250fold increase of catalytic activity at 2 mM | Caenorhabditis elegans | |
spermine | 90fold increase of catalytic activity at 2 mM | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
D190N | reduced activation by putrescine, spermidine or spermine | Caenorhabditis elegans |
E194Q | strongly enhanced basic activity, little activation by putrescine, spermidine or spermine | Caenorhabditis elegans |
E274Q | slightly enhanced basic activity, reduced activation by putrescine, spermidine or spermine | Caenorhabditis elegans |
E33Q | drastically reduced activation by putrescine, no activation by spermidine or spermine, no complete processing of pro-protein | Caenorhabditis elegans |
K95A | no activation, no complete processing of pro-protein | Caenorhabditis elegans |
R91A | drastically reduced activation by putrescine, spermidine or spermine, no complete processing of pro-protein | Caenorhabditis elegans |
S128A | drastically reduced activation by putrescine, spermidine or spermine | Caenorhabditis elegans |
S83A | no activation, no processing of pro-protein | Caenorhabditis elegans |
T192A | slightly enhanced basic activity, reduced activation by putrescine, spermidine or spermine | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.115 | - |
S-adenosyl-L-methionine | in presence of 2 mM putrescine | Caenorhabditis elegans | |
0.47 | - |
S-adenosyl-L-methionine | in presence of 0.5 mM putrescine | Caenorhabditis elegans | |
1 | - |
S-adenosyl-L-methionine | in presence of 0.3 mM putrescine | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | - |
Caenorhabditis elegans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0005 | - |
without activator | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | - |
Caenorhabditis elegans | (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2 | - |
? |