Cloned (Comment) | Organism |
---|---|
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells | Ruminococcus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
phosphoenolpyruvate | in 50 mM HEPES (pH 7.0) | Ruminococcus albus | |
0.81 | - |
ADP | in 50 mM HEPES (pH 7.0) | Ruminococcus albus | |
1.81 | - |
oxaloacetate | in 50 mM HEPES (pH 7.0) | Ruminococcus albus | |
1.83 | - |
ATP | in 50 mM HEPES (pH 7.0) | Ruminococcus albus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 100% activity in the presence of 10 mM Mg2+ | Ruminococcus albus | |
Mn2+ | 85.3% activity in the presence of 10 mM Mn2+ | Ruminococcus albus | |
additional information | not activated by Co2+, Ni2+, Cu2+, or Zn2+ | Ruminococcus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59430 | - |
calculated from amino acid sequence | Ruminococcus albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus albus | B3Y6D3 | strain ATCC27210[T] | - |
Ruminococcus albus ATCC 27210[T] | B3Y6D3 | strain ATCC27210[T] | - |
Purification (Comment) | Organism |
---|---|
HiTrap chelating affinity column chromatography and Superdex 200 gel filtration | Ruminococcus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxaloacetate | - |
Ruminococcus albus | ADP + phosphoenolpyruvate + CO2 | ADP is the preferred PCK phosphate acceptor | r | |
ATP + oxaloacetate | - |
Ruminococcus albus ATCC 27210[T] | ADP + phosphoenolpyruvate + CO2 | ADP is the preferred PCK phosphate acceptor | r | |
GTP + oxaloacetate | when ADP is replaced by GDP in the PEP-carboxylating reaction, PCK activity decreases approximately 5fold | Ruminococcus albus | GDP + phosphoenolpyruvate + CO2 | - |
r | |
GTP + oxaloacetate | when ADP is replaced by GDP in the PEP-carboxylating reaction, PCK activity decreases approximately 5fold | Ruminococcus albus ATCC 27210[T] | GDP + phosphoenolpyruvate + CO2 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PCK | - |
Ruminococcus albus |
phosphoenolpyruvate carboxykinase | - |
Ruminococcus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Ruminococcus albus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Ruminococcus albus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | preferred cofactor | Ruminococcus albus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ruminococcus albus | calculated from amino acid sequence | - |
4.9 |