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Literature summary for 4.1.1.49 extracted from
- Relevance of Arg457 for the nucleotide affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase (2008), Int. J. Biochem. Cell Biol., 40, 1883-1889.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transformed into the PEP carboxykinase-deficient yeast strain PUK-3B (MATalpha pck ura3) | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R457M | site directed mutagenesis | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
CO2 | for wild-type | Saccharomyces cerevisiae |  |
| 0.009 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
| 0.0098 | - |
CO2 | for R457M mutant | Saccharomyces cerevisiae | |
| 0.0098 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
| 0.04 | - |
ADP | for wild-type | Saccharomyces cerevisiae | |
| 0.04 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
| 0.3 | - |
phosphoenolpyruvate | for R457M mutant | Saccharomyces cerevisiae | |
| 0.31 | - |
phosphoenolpyruvate | for wild-type | Saccharomyces cerevisiae | |
| 2.03 | - |
ADP | for R457M mutant | Saccharomyces cerevisiae | |
| 2.03 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae | |
| Mn2+ | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 251000 | - |
calculated | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P10963 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SDS-PAGE | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate + CO2 | - |
Saccharomyces cerevisiae | ATP + oxaloacetate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP: oxaloacetate carboxy-lyase | - |
Saccharomyces cerevisiae |
| PEP carboxykinase | - |
Saccharomyces cerevisiae |
| phosphoenolpyruvate carboxykinase | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | 6 | phosphoenolpyruvate | for wild-type | Saccharomyces cerevisiae | |
| 13 | - |
phosphoenolpyruvate | for R457M mutant | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | preference for ADP (or ATP) over other nucleotides | Saccharomyces cerevisiae | |
| ATP | preference for ADP (or ATP) over other nucleotides | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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