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Literature summary for 4.1.1.49 extracted from
- Functional evaluation of serine 252 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase (2008), Biochimie, 91, 295-299.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant plasmid is transformed into the phosphoenolpyruvate carboxykinase-deficient yeast strain PUK-3B (MATalpha pck ura3) | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S252A | side directed mutagenesis | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | MnATP2- as substrate, for S252A mutant Km above 3000 microM | Saccharomyces cerevisiae | |
| 0.026 | - |
oxaloacetate | S252A mutant | Saccharomyces cerevisiae |  |
| 0.03 | - |
oxaloacetate | wild-type | Saccharomyces cerevisiae | |
| 0.034 | - |
ADP | wild-type | Saccharomyces cerevisiae | |
| 0.034 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
| 0.037 | - |
phosphoenolpyruvate | for the pyruvate kinase-like activity of S252A mutant | Saccharomyces cerevisiae | |
| 0.067 | - |
ATP | wild-type | Saccharomyces cerevisiae | |
| 0.067 | - |
ATP | ATP in form of MnATP2- | Saccharomyces cerevisiae | |
| 0.077 | - |
ADP | for the pyruvate kinase-like activity of S252A mutant | Saccharomyces cerevisiae | |
| 0.077 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
| 0.082 | - |
phosphoenolpyruvate | S252A mutant | Saccharomyces cerevisiae | |
| 0.122 | - |
oxalacetate | for the oxaloacetate decarboxylase activity of wild-type | Saccharomyces cerevisiae | |
| 0.132 | - |
ADP | S252A mutant | Saccharomyces cerevisiae | |
| 0.132 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
| 0.135 | - |
phosphoenolpyruvate | for the pyruvate kinase-like activity of wild-type | Saccharomyces cerevisiae | |
| 0.151 | - |
ADP | for the pyruvate kinase-like activity of wild-type | Saccharomyces cerevisiae | |
| 0.151 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
| 0.307 | - |
phosphoenolpyruvate | wild-type | Saccharomyces cerevisiae | |
| 5.2 | - |
oxalacetate | for the oxaloacetate decarboxylase activity of S252A mutant | Saccharomyces cerevisiae | |
| 18 | - |
CO2 | wild-type | Saccharomyces cerevisiae | |
| 18 | - |
CO2 | S252A mutant | Saccharomyces cerevisiae | |
| 18 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P10963 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate + CO2 | - |
Saccharomyces cerevisiae | ATP + oxaloacetate | - |
r | |
| ATP + oxaloacetate | interacts also with GTP, EC 4.1.1.32 | Saccharomyces cerevisiae | ADP + phosphoenolpyruvate + CO2 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP:oxaloacetate carboxy-lyase | transphosphorylating, interacts also with GTP, EC 4.1.1.32 | Saccharomyces cerevisiae |
| PEP carboxykinase | - |
Saccharomyces cerevisiae |
| phosphoenolpyruvate carboxykinase | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat for oxalacetate as a substrate is above 19 1/s for S252A mutant in the phosphoenolpyruvate formation direction | Saccharomyces cerevisiae | |
| 0.2 | - |
phosphoenolpyruvate | for the pyruvate kinase-like activity of S252A mutant | Saccharomyces cerevisiae | |
| 0.32 | - |
oxalacetate | for the oxaloacetate decarboxylase activity of wild-type | Saccharomyces cerevisiae | |
| 0.5 | - |
phosphoenolpyruvate | S252A mutant, apparent kinetic parameters for S252A mutant in the phosphoenolpyruvate carboxylation direction | Saccharomyces cerevisiae | |
| 0.8 | - |
phosphoenolpyruvate | for the pyruvate kinase-like activity of wild-type | Saccharomyces cerevisiae | |
| 1 | - |
oxalacetate | for the oxaloacetate decarboxylase activity of S252A mutant | Saccharomyces cerevisiae | |
| 62 | - |
phosphoenolpyruvate | wild-type, apparent kinetic parameters for wild-type in the phosphoenolpyruvate carboxylation direction | Saccharomyces cerevisiae | |
| 65 | - |
oxalacetate | wild-type, apparent kinetic parameters for wild-type in the phosphoenolpyruvate formation direction | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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