Protein Variants | Comment | Organism |
---|---|---|
R70K | 1.7fold increase in KM-value for ADP, 1.3fold increase in KM-value for phosphoenolpyruvate, 1.4fold increase in KM-value for HCO3- | Saccharomyces cerevisiae |
R70M | 2.5fold increase in KM-value for ADP, 2.4fold increase in KM-value for phosphoenolpyruvate, 1.8fold decrease in KM-value for HCO3- | Saccharomyces cerevisiae |
R70Q | 1.9fold increase in KM-value for ADP, 2.3fold increase in KM-value for phosphoenolpyruvate, 1.1fold increase in KM-value for HCO3- | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
CO2 | pH 6.6, 30°C, mutant enzyme R70M | Saccharomyces cerevisiae | |
0.01 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
0.018 | - |
CO2 | pH 6.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.018 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
0.019 | - |
CO2 | pH 6.6, 30°C, mutant enzyme R70Q | Saccharomyces cerevisiae | |
0.019 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
0.025 | - |
CO2 | pH 6.6, 30°C, mutant enzyme R70K | Saccharomyces cerevisiae | |
0.025 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
0.034 | - |
ADP | pH 6.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.058 | - |
ADP | pH 6.6, 30°C, mutant enzyme R70K | Saccharomyces cerevisiae | |
0.063 | - |
ADP | pH 6.6, 30°C, mutant enzyme R70Q | Saccharomyces cerevisiae | |
0.086 | - |
ADP | pH 6.6, 30°C, mutant enzyme R70M | Saccharomyces cerevisiae | |
0.307 | - |
phosphoenolpyruvate | pH 6.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.41 | - |
phosphoenolpyruvate | pH 6.6, 30°C, mutant enzyme R70K | Saccharomyces cerevisiae | |
0.72 | - |
phosphoenolpyruvate | pH 6.6, 30°C, mutant enzyme R70Q | Saccharomyces cerevisiae | |
0.73 | - |
phosphoenolpyruvate | pH 6.6, 30°C, mutant enzyme R70M | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate + CO2 | Arg70 is important for catalytic efficiency of the enzyme and for binding of phosphoenolpyruvate | Saccharomyces cerevisiae | ATP + oxaloacetate | - |
? |