Literature summary for 4.1.1.48 extracted from

Zaccardi, M.J., O'Rourke, K.F.; Yezdimer, E.M.; Loggia, L.J.; Woldt, S.; Boehr, D.D.
Loop-loop interactions govern multiple steps in indole-3-glycerol phosphate synthase catalysis (2014), Protein Sci., 23, 302-311.

Search for more literature for 4.1.1.48

Protein Variants

Protein Variants	Comment	Organism
F89A	approximately 24fold increase in the KM-value, eleven-fold decrease in the maximum turnover rate	Saccharolobus solfataricus
N90A	loss of the descending limb in the pH rate profile, 5-fold decrease in kcat at 25°C, but at higher temperatures (i.e. 75°C), the kinetic parameters for the N90A mutant enzyme closer to wild-type enzyme. Mutant enzyme has substantially reduced solvent deuterium kinetic isotope effects compared to wild-type enzyme at 75°C. 2fold decrease in the rate of thermal inactivation at 90°C as compared to wild-type enzyme	Saccharolobus solfataricus
N90Q	the steady-state kinetics for the mutant enzyme are substantially decreased compared to wild-type enzyme at lower temperatures, but kcat of the N90Q variant approaches that of wild-type enzyme at higher temperatures. The solvent deuterium kinetic isotope effects on kcat is also substantially reduced compared to wild-type enzyme. 2fold decrease in the rate of thermal inactivation at 90°C	Saccharolobus solfataricus
R54A	mutation does not result in any substantial change to the steady-state kinetic parameters at any of the temperatures assayed (25°C, 37°C, 75°C). At 75°C, the mutant enzyme shows a small increase to kcat (about 1.8 fold) compared to wild-type enzyme. Mutant enzyme has substantially reduced solvent deuterium kinetic isotope effects compared to wild-type enzyme at 75°C. Thermal inactivation constant at 90°C is similar to wild-typ enzyme	Saccharolobus solfataricus
R54A/N90A	the R54A/N90A double substitution are not additive with the effects of the R54A and N90A single substitutions suggesting some type of thermodynamic coupling between these residues	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000023	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme R54A/N90Q	Saccharolobus solfataricus
0.000036	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, mutant enzyme N90A	Saccharolobus solfataricus
0.000044	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, wild-type enzyme	Saccharolobus solfataricus
0.000074	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, wild-type enzyme	Saccharolobus solfataricus
0.000074	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme N90A	Saccharolobus solfataricus
0.000074	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme R54A	Saccharolobus solfataricus
0.000079	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme N90A	Saccharolobus solfataricus
0.000088	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 27°C, wild-type enzyme	Saccharolobus solfataricus
0.000095	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme R54A	Saccharolobus solfataricus
0.000107	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme R54A/N90Q	Saccharolobus solfataricus
0.000117	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme N90Q	Saccharolobus solfataricus
0.000132	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme N90Q	Saccharolobus solfataricus
0.000145	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, mutant enzyme R54A	Saccharolobus solfataricus
0.0012	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme F89A	Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	Saccharolobus solfataricus	tryptophan biosynthetic enzyme	1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	-	Saccharolobus solfataricus	1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O	-	?
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	tryptophan biosynthetic enzyme	Saccharolobus solfataricus	1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
IGPS	-	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	mutant enzyme N90A shows 2fold decrease in the rate of thermal inactivation at 90°C as compared to wild-type enzyme. Thermal inactivation constant of muta t enzymes N90A or N90Q at 90°C is similar to wild-type enzyme	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.04	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, mutant enzyme N90A	Saccharolobus solfataricus
0.06	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme F89A	Saccharolobus solfataricus
0.16	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, wild-type enzyme	Saccharolobus solfataricus
0.16	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, mutant enzyme R54A	Saccharolobus solfataricus
0.17	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme N90A	Saccharolobus solfataricus
0.19	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme R54A/N90Q	Saccharolobus solfataricus
0.3	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme N90Q	Saccharolobus solfataricus
0.35	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme R54A	Saccharolobus solfataricus
0.42	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, wild-type enzyme	Saccharolobus solfataricus
0.51	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme R54A/N90Q	Saccharolobus solfataricus
0.55	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme N90A	Saccharolobus solfataricus
0.67	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, wild-type enzyme	Saccharolobus solfataricus
0.89	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme N90Q	Saccharolobus solfataricus
1.2	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme R54A	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
50	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme F89A	Saccharolobus solfataricus
1000	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, mutant enzyme N90A	Saccharolobus solfataricus
2200	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, mutant enzyme R54A	Saccharolobus solfataricus
2200	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 25°C, wild-type enzyme	Saccharolobus solfataricus
2300	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme N90A	Saccharolobus solfataricus
2300	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme N90Q	Saccharolobus solfataricus
3700	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme R54A	Saccharolobus solfataricus
4800	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, wild-type enzyme	Saccharolobus solfataricus
4800	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme R54A/N90Q	Saccharolobus solfataricus
7000	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme N90A	Saccharolobus solfataricus
7600	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme N90Q	Saccharolobus solfataricus
8200	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 37°C, mutant enzyme R54A/N90Q	Saccharolobus solfataricus
15000	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, wild-type enzyme	Saccharolobus solfataricus
16000	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	pH 7.0, 75°C, mutant enzyme R54A	Saccharolobus solfataricus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)