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Literature summary for 4.1.1.48 extracted from

  • Merz, A.; Yee, M.C.; Szadkowski, H.; Pappenberger, G.; Crameri, A.; Stemmer, W.P.; Yanofsky, C.; Kirschner, K.
    Improving the catalytic activity of a thermophilic enzyme at low temperatures (2000), Biochemistry, 39, 880-889.
    View publication on PubMed
Search for more literature for 4.1.1.48

Protein Variants

Protein Variants Comment Organism
F246S about 2fold increase in turnover-number, 8fold increase in KM-value, decrease in kcat/Km Saccharolobus solfataricus
G212E about 2fold increase in turnover-number, 100fold increase Km-value, decrease in kcat/Km. Mutant enzyme is about as thermostable as wild-type enzyme Saccharolobus solfataricus
M237T kcat/Km of 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate is fold than wild-type value Saccharolobus solfataricus
P2S slight increase in kcat/Km (substrate: 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate). Mutant enzyme is about as thermostable as wild-type enzyme Saccharolobus solfataricus
P2S/F246S about 2fold increase in turnover-number, 8fold increase in KM-value, decrease in kcat/Km Saccharolobus solfataricus
P2S/G212E about 4fold increase in turnover-number,200fold increase in Km-valuer, decrease in kcat/Km Saccharolobus solfataricus

General Stability

General Stability Organism
half-life after trypsinolysis in 0.1 M Tris acetate at pH 7.8 and 25°C: wild-type enzyme (120 min), mutant enzyme P2S (60 min), mutant enzyme F246S (40 min), mutant enzyme G212E (40 min), mutant enzyme P2S/F246S (8 min), mutant enzyme P2S/G212E (15 min) Saccharolobus solfataricus
the enzyme is strongly stabilized in phosphate buffer (t1/2: 46 min at 87°C) in comparison to HEPPS buffer (t1/2: 4.4 min at 89°C) Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, wild-type enzyme Saccharolobus solfataricus
0.00005
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, wild-type enzyme Saccharolobus solfataricus
0.00005
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme P2S Saccharolobus solfataricus
0.00005
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 60°C, wild-type enzyme Saccharolobus solfataricus
0.0004
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme F246S Saccharolobus solfataricus
0.00042
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, mutant enzyme P2S/F246S Saccharolobus solfataricus
0.0056
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme G212E Saccharolobus solfataricus
0.0109
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, mutant enzyme P2S/G212E Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Saccharolobus solfataricus indoleglycerol phosphate + CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
IGP synthase
-
 Saccharolobus solfataricus
IGPS
-
 Saccharolobus solfataricus
Indoleglycerol phosphate synthase
-
 Saccharolobus solfataricus
TrpC
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
86.5
-
 in 0.05 M potassium phosphate at pH 7.5. Half-life of wild-type enzyme: 46 min, half-life of mutant enzyme P2S: 18 min, hal-life of mutant enzyme F246S 1 min, half-life of mutant enzyme G212E: 38 min, half-life of mutant enzyme P2S/F246S: less than 0.1 min, half-life of mutant enzyme P2S/G212E: 19 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, wild-type enzyme Saccharolobus solfataricus
0.15
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, wild-type enzyme Saccharolobus solfataricus
0.16
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme P2S Saccharolobus solfataricus
0.27
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, mutant enzyme P2S/F246S Saccharolobus solfataricus
0.35
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme F246S Saccharolobus solfataricus
0.36
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme G212E Saccharolobus solfataricus
0.57
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, mutant enzyme P2S/G212E Saccharolobus solfataricus
0.98
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 60°C, wild-type enzyme Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
100
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, mutant enzyme P2S/G212E Saccharolobus solfataricus
100
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme G212E Saccharolobus solfataricus
600
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, mutant enzyme P2S/F246S Saccharolobus solfataricus
750
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C, wild-type enzyme Saccharolobus solfataricus
900
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme F246S Saccharolobus solfataricus
3000
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, wild-type enzyme Saccharolobus solfataricus
3200
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 37°C, mutant enzyme P2S Saccharolobus solfataricus
14200
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 60°C, wild-type enzyme Saccharolobus solfataricus