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Literature summary for 4.1.1.48 extracted from

  • Schlee, S.; Dietrich, S.; Kurcon, T.; Delaney, P.; Goodey, N.M.; Sterner, R.
    Kinetic mechanism of indole-3-glycerol phosphate synthase (2012), Biochemistry, 52, 132-142.
    View publication on PubMed
Search for more literature for 4.1.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
mutagenesis data and crystal structure analysis of IGPS from Sulfolobus solfataricus allows for the formulation of a plausible chemical mechanism of the reaction Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D61C kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is about 1.5fold lower than the wild-type value Saccharolobus solfataricus
R18C kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is about 1.5fold lower than the wild-type value Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000045
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye Alexa Fluor 555 Saccharolobus solfataricus
0.000046
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye PyMPO Saccharolobus solfataricus
0.00005
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye PyMPO Saccharolobus solfataricus
0.000051
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, wild-type enzyme Saccharolobus solfataricus
0.000109
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye Alexa Fluor 555 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate Saccharolobus solfataricus the enzyme is involved in tryptophan biosynthesis 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
 ?
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate Saccharolobus solfataricus P2 the enzyme is involved in tryptophan biosynthesis 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q06121
-
-
Saccharolobus solfataricus P2 Q06121
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate the enzyme is involved in tryptophan biosynthesis Saccharolobus solfataricus 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
 ?
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate elucidation of the kinetic reaction mechanism, importance of flexible active loops for substrate binding and catalysis by the enzyme, product release is the rate-limiting step of the overall reaction Saccharolobus solfataricus 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
 ?
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate the enzyme is involved in tryptophan biosynthesis Saccharolobus solfataricus P2 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
 ?
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate elucidation of the kinetic reaction mechanism, importance of flexible active loops for substrate binding and catalysis by the enzyme, product release is the rate-limiting step of the overall reaction Saccharolobus solfataricus P2 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
sIGPS
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye PyMPO Saccharolobus solfataricus
0.04
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye Alexa Fluor 555 Saccharolobus solfataricus
0.042
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye PyMPO Saccharolobus solfataricus
0.062
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, wild-type enzyme Saccharolobus solfataricus
0.065
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye Alexa Fluor 555 Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
596
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye Alexa Fluor 555 Saccharolobus solfataricus
826
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye PyMPO Saccharolobus solfataricus
840
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye PyMPO Saccharolobus solfataricus
888
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye Alexa Fluor 555 Saccharolobus solfataricus
1215
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C, wild-type enzyme Saccharolobus solfataricus