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Literature summary for 4.1.1.48 extracted from

  • Merz, A.; Knochel, T.; Jansonius, J.N.; Kirschner, K.
    The hyperthermostable indoleglycerol phosphate synthase from Thermotoga maritima is destabilized by mutational disruption of two solvent-exposed salt bridges (1999), J. Mol. Biol., 288, 753-763.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D184A no alteration of native structure, very little difference in Km, turnover rate compared to wild type, but less thermal stability Thermotoga maritima
R241A no alteration of native structure, very little difference in Km, turnover rate compared to wild type, but less thermal stability Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000006
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 25°C Thermotoga maritima
0.000014
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 45°C Thermotoga maritima
0.000053
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 60°C Thermotoga maritima
0.000123
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate pH 7.5, 80°C Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate Thermotoga maritima
-
1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
ir

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
Thermotoga maritima 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
73
-
335 min, half-life for irreversible thermal inactivation Thermotoga maritima
83.5
-
110 min, half-life for irreversible thermal inactivation Thermotoga maritima
85.5
-
14.4 min, half-life for irreversible thermal inactivation, mutant R241A Thermotoga maritima
85.5
-
36.1 min, half-life for irreversible thermal inactivation, mutant D184A Thermotoga maritima
85.5
-
42.3 min, half-life for irreversible thermal inactivation Thermotoga maritima
90
-
11 min, half-life for irreversible thermal inactivation Thermotoga maritima