Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering of a monofunctional enzyme by separating the domains on the gene level and expression as monofunctional enzymes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Reduced 1-[2-carboxyphenyl)amino]-1-deoxyribulose 5-phosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000047 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | genetically engineered monofunctional enzyme form IGPS[1-259] | Escherichia coli | |
0.00042 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | bifunctional enzyme | Escherichia coli | |
0.013 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | genetically engineered monofunctional enzyme form IGPS[1-252FAG] | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
bifunctional enzyme phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase, Ec4.1.1.48/Ec5.3.1.24 | - |
Purification (Comment) | Organism |
---|---|
monofunctional enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Escherichia coli | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | genetically engineered monofunctional enzyme form IGPS[1-259] | Escherichia coli | |
3.6 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | bifunctional enzyme | Escherichia coli | |
4.3 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | genetically engineered monofunctional enzyme form IGPS[1-252FAG] | Escherichia coli |