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Literature summary for 4.1.1.46 extracted from

  • Sun, X.; Lin, Y.; Yuan, Q.; Yan, Y.
    Biological production of muconic acid via a prokaryotic 2,3-dihydroxybenzoic acid decarboxylase (2014), ChemSusChem, 7, 2478-2481 .
    View publication on PubMed
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Application

Application Comment Organism
synthesis construction of an artificial pathway for muconic acid production in Escherichia coli by connecting 2,3-dihydroxybenzoic acid biosynthesis with its degradation pathway. Overexpression of entCBA and the key enzymes in the shikimate pathway leads to the production of 900 mg/l of 2,3-dihydroxybenzoic acid. Expression of 2,3-dihydroxybenzoic acid decarboxylase coupled with catechol 1,2-dioxygenase achieves the conversion of 2,3-dihydroxybenzoic acid into muconic acid. Assembly of the total pathway results in the de novo production of muconic acid up to 480 mg/l Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.42
-
 2,3-Dihydroxybenzoate pH 5.0, 30°C Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-Dihydroxybenzoate
-
 Klebsiella pneumoniae Catechol + CO2
-
 ?
2,4-dihydroxybenzoate
-
 Klebsiella pneumoniae 3-hydroxyphenol + CO2
-
 ?
2,5-dihydroxybenzoate
-
 Klebsiella pneumoniae 4-hydroxyphenol + CO2
-
 ?
additional information no substrate: 2,4-dihydroxybenzoate Klebsiella pneumoniae ?
-
 ?
salicylic acid
-
 Klebsiella pneumoniae phenol + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE, x * 38400, calculated from sequence, recombinant protein with His-tag Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.065
-
 2,3-Dihydroxybenzoate pH 5.0, 30°C Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor required Klebsiella pneumoniae