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Literature summary for 4.1.1.45 extracted from

  • Li, T.; Iwaki, H.; Fu, R.; Hasegawa, Y.; Zhang, H.; Liu, A.
    Alpha-amino-beta-carboxymuconic-epsilon-semialdehyde decarboxylase (ACMSD) is a new member of the amidohydrolase superfamily (2006), Biochemistry, 45, 6628-6634.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
D294E the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure Pseudomonas fluorescens
H177A the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure Pseudomonas fluorescens
H228A the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure Pseudomonas fluorescens
H228E the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure Pseudomonas fluorescens
H9E the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme H228A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.0123
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme H177A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.0167
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate wild type enzyme, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.0241
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme H9E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.0662
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme H228E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.0672
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme D294E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA superflow resin chromatography and HisPrep FF column chromatography Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate
-
Pseudomonas fluorescens 2-aminomuconate-6-semialdehyde + CO2
-
?

Synonyms

Synonyms Comment Organism
ACMSD
-
Pseudomonas fluorescens
alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase
-
Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.019
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme H228E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.025
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme H9E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.08
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme D294E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.11
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme H228A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
0.28
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate mutant enzyme H177A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens
6.65
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate wild type enzyme, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C Pseudomonas fluorescens