Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
D294E | the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure | Pseudomonas fluorescens |
H177A | the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure | Pseudomonas fluorescens |
H228A | the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure | Pseudomonas fluorescens |
H228E | the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure | Pseudomonas fluorescens |
H9E | the mutation causes a dramatic loss of enzyme activity, substantial reduction of the metal-binding ability, and an altered metallocenter electronic structure | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme H228A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.0123 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme H177A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.0167 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | wild type enzyme, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.0241 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme H9E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.0662 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme H228E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.0672 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme D294E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA superflow resin chromatography and HisPrep FF column chromatography | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | - |
Pseudomonas fluorescens | 2-aminomuconate-6-semialdehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACMSD | - |
Pseudomonas fluorescens |
alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase | - |
Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme H228E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.025 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme H9E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.08 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme D294E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.11 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme H228A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
0.28 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | mutant enzyme H177A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens | |
6.65 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | wild type enzyme, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C | Pseudomonas fluorescens |