Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method with 0.1 M Tris (pH 8.75), 15% PEG 5000, and 0.2 M MgCl2, at 20°C | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
H177A | similar properties like the wild type enzyme | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | in 25 mM HEPES buffer pH 7.0, the enzyme contains Zn2+ | Pseudomonas fluorescens | |
0.0232 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | in 25 mM HEPES buffer pH 7.0, the enzyme contains Co2+ | Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Pseudomonas fluorescens | |
Zn2+ | native active site metal | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose HP column chromatography, Phenyl Sepharose column chromatography | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | - |
Pseudomonas fluorescens | 2-aminomuconate-6-semialdehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | the protein exists in solution as a mixture of both monomer and dimer forms | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
ACMSD | member of the metal-dependent amidohydrolase superfamily of the (beta/alpha)8 TIM barrel fold | Pseudomonas fluorescens |
alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase | - |
Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | in 25 mM HEPES buffer pH 7.0, the enzyme contains Zn2+ | Pseudomonas fluorescens | |
7.3 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | in 25 mM HEPES buffer pH 7.0, the enzyme contains Co2+ | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Pseudomonas fluorescens |