Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.44 extracted from

  • Lee, H.Y.; Yang, J.K.
    Crystallization and preliminary X-ray crystallographic analysis of gamma-carboxymucolactone decarboxylase from Sulfolobus solfataricus (2009), Acta Crystallogr. Sect. F, 65, 1197-1199.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain Rosetta 2 (DE3) Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method, 22%(w/v) polyethylene glycol 3350, 0.2 M sodium chloride, 0.1 M Na HEPES pH 7.0 and 10%(v/v) glycerol, the crystal belongs to space group P43212, with unit-cell parameters a = b = 66.66, c = 184.82 A, resolution 2.40 A, the Matthews coefficient and solvent content are estimated to be 2.14 A3/Da and 42.6%, respectively, assuming that the asymmetric unit contains three recombinant protein molecules Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15950
-
caculated value Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-carboxymuconolactone Saccharolobus solfataricus
-
beta-oxoadipate enol-lactone + ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
centrifugal ultrafiltration, gel filtration Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxymuconolactone
-
Saccharolobus solfataricus beta-oxoadipate enol-lactone + ?
-
?

Synonyms

Synonyms Comment Organism
gamma-carboxymuconolactone decarboxylase
-
Saccharolobus solfataricus
gamma-CMD
-
Saccharolobus solfataricus