Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain Rosetta 2 (DE3) | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, 22%(w/v) polyethylene glycol 3350, 0.2 M sodium chloride, 0.1 M Na HEPES pH 7.0 and 10%(v/v) glycerol, the crystal belongs to space group P43212, with unit-cell parameters a = b = 66.66, c = 184.82 A, resolution 2.40 A, the Matthews coefficient and solvent content are estimated to be 2.14 A3/Da and 42.6%, respectively, assuming that the asymmetric unit contains three recombinant protein molecules | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15950 | - |
caculated value | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxymuconolactone | Saccharolobus solfataricus | - |
beta-oxoadipate enol-lactone + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
centrifugal ultrafiltration, gel filtration | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxymuconolactone | - |
Saccharolobus solfataricus | beta-oxoadipate enol-lactone + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-carboxymuconolactone decarboxylase | - |
Saccharolobus solfataricus |
gamma-CMD | - |
Saccharolobus solfataricus |