Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E545L | the mutant shows 730fold decrease in kcat (phenylpyruvate) and 38fold decrease in kcat (indole-3-pyruvate) compared to the wild type enzyme | Saccharomyces cerevisiae |
I335Y | compared to the wild type enzyme, the mutant exhibits 0.6% catalytic efficiency with phenylpyruvate, 33% catalytic efficiency with 2-oxohexanoic acid, 213% catalytic efficiency with 2-oxopentanoic acid, 1.7% catalytic efficiency with 2-oxobutanoic acid, and 7% catalytic efficiency with pyruvic acid | Saccharomyces cerevisiae |
I335Y/M624W | compared to the wild type enzyme, the mutant exhibits 0.02% catalytic efficiency with phenylpyruvate, 2.3% catalytic efficiency with 2-oxohexanoic acid, 25% catalytic efficiency with 2-oxopentanoic acid, no activity with 2-oxobutanoic acid, and 59% catalytic efficiency with pyruvic acid | Saccharomyces cerevisiae |
M624W | compared to the wild type enzyme, the mutant exhibits 3.4% catalytic efficiency with phenylpyruvate, 32% catalytic efficiency with 2-oxohexanoic acid, 131% catalytic efficiency with 2-oxopentanoic acid, 1.4% catalytic efficiency with 2-oxobutanoic acid, and 152% catalytic efficiency with pyruvic acid | Saccharomyces cerevisiae |
Q448W | compared to the wild type enzyme, the mutant exhibits 45% catalytic efficiency with phenylpyruvate, 16% catalytic efficiency with 2-oxohexanoic acid, 231% catalytic efficiency with 2-oxopentanoic acid, 62% catalytic efficiency with 2-oxobutanoic acid, and 130% catalytic efficiency with pyruvic acid | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
Indole-3-pyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.054 | - |
phenylpyruvate | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.09 | - |
4-hydroxyphenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.09 | - |
2-oxo-4-phenylbutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.1 | - |
phenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.17 | - |
2-oxohexanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.18 | - |
phenylpyruvate | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.37 | - |
2-oxopentanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.46 | - |
2-oxohexanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.54 | - |
2-oxopentanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.55 | - |
2-oxohexanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.63 | - |
2-oxopentanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.64 | - |
4-methylthio-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.69 | - |
2-oxohexanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.8 | - |
phenylpyruvate | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.9 | - |
4-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.96 | - |
2-oxopentanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
1 | - |
2-oxohexanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
2.1 | - |
2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
3.1 | - |
3-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
5.5 | - |
2-oxobutanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
5.5 | - |
Pyruvic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
6.4 | - |
2-oxobutanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
6.4 | - |
Pyruvic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
7 | - |
2-oxobutanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
7.6 | - |
2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
8.5 | - |
3-methyl-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
9.7 | - |
Pyruvic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
25 | - |
Pyruvic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q06408 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-phenylbutanoic acid | 9% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
2-oxobutanoic acid | 0.2% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
2-oxohexanoic acid | 6% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
2-oxopentanoic acid | 1% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
3-methyl-2-oxobutanoic acid | 1% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
3-methyl-2-oxopentanoic acid | 2% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4-hydroxyphenylpyruvate | 63% activity compared to phenylpyruvate | Saccharomyces cerevisiae | 4-hydroxyphenylacetaldehyde + CO2 | - |
? | |
4-methyl-2-oxopentanoic acid | 6% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4-methylthio-2-oxobutanoic acid | 6% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
benzoylformic acid | 0.2% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
indole-3-pyruvate | preferred substrate, 100% activity | Saccharomyces cerevisiae | indoleacetaldehyde + CO2 | - |
? | |
additional information | PPDC is essentially unable to utilize pyruvate and 3-indoleglyoxylic acid | Saccharomyces cerevisiae | ? | - |
? | |
Phenylpyruvate | preferred substrate, 100% activity | Saccharomyces cerevisiae | Phenylacetaldehyde + CO2 | - |
? | |
pyruvic acid | 0.02% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
phenylpyruvate decarboxylase | - |
Saccharomyces cerevisiae |
PPDC | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2-oxobutanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.06 | - |
2-oxobutanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.064 | - |
Pyruvic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.14 | - |
Indole-3-pyruvate | mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.32 | - |
Pyruvic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.34 | - |
Pyruvic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.39 | - |
2-oxopentanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.41 | - |
2-oxohexanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.45 | - |
Pyruvic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.76 | - |
2-oxopentanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.85 | - |
phenylpyruvate | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.96 | - |
2-oxohexanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
1.2 | - |
phenylpyruvate | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
1.3 | - |
2-oxohexanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
1.7 | - |
2-oxo-4-phenylbutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
2 | - |
2-oxopentanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
2.1 | - |
2-oxopentanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
2.2 | - |
2-oxobutanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
3.3 | - |
2-oxohexanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
3.9 | - |
2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.8 | - |
phenylpyruvate | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
5.2 | - |
2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
5.4 | - |
Indole-3-pyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
7.7 | - |
4-methylthio-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
8.8 | - |
phenylpyruvate | mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
8.8 | - |
2-oxohexanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
10 | - |
4-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
11 | - |
4-hydroxyphenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
11 | - |
3-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
19 | - |
3-methyl-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
20 | - |
phenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Pyruvic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.007 | - |
2-oxobutanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.009 | - |
2-oxobutanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.027 | - |
Pyruvic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.03 | - |
phenylpyruvate | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.035 | - |
Pyruvic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.06 | - |
Pyruvic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.07 | - |
Pyruvic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.315 | - |
2-oxobutanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.35 | - |
benzoylformic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.4 | - |
2-oxopentanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.42 | - |
2-oxohexanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
0.52 | - |
2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
1.1 | - |
phenylpyruvate | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
2.1 | - |
2-oxopentanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
2.2 | - |
3-methyl-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
2.5 | - |
2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
2.8 | - |
2-oxohexanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
3.4 | - |
2-oxopentanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
3.5 | - |
3-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
3.7 | - |
2-oxopentanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
5.8 | - |
2-oxohexanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
6 | - |
2-oxohexanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
11 | - |
4-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
12 | - |
4-methylthio-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
13 | - |
2-oxohexanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
19 | - |
2-oxo-4-phenylbutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
125 | - |
4-hydroxyphenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
200 | - |
phenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
200 | - |
Indole-3-pyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae |