Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | activates about 2fold at 5-10 mM | Clostridium acetobutylicum | |
methyl viologen | activates 6.7fold at 10 mM | Clostridium acetobutylicum |
Application | Comment | Organism |
---|---|---|
synthesis | acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid in an enzymatic system for synthesis of 2-butanone from levulinic acid | Clostridium acetobutylicum |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), best induction over 24 h at 20°C with 1 mM IPTG | Clostridium acetobutylicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Azure B | slight inhibition at 2-10 mM | Clostridium acetobutylicum | |
levulinic acid | substrate inhibition is observed with levulinic acid concentration higher than 5 mM | Clostridium acetobutylicum | |
methylene blue | complete inhibition at 2 mM | Clostridium acetobutylicum | |
Zn2+ | inhibits at above 5 mM | Clostridium acetobutylicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetate + H+ | Clostridium acetobutylicum | - |
acetone + CO2 | - |
? | |
additional information | Clostridium acetobutylicum | acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | P23670 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Clostridium acetobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetate + H+ | - |
Clostridium acetobutylicum | acetone + CO2 | - |
? | |
levulinic acid + H+ | - |
Clostridium acetobutylicum | 2-butanone + CO2 | - |
? | |
additional information | acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid | Clostridium acetobutylicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AADC | - |
Clostridium acetobutylicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Clostridium acetobutylicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | over 30% of maximal activity at 20°C and 45°C | Clostridium acetobutylicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
with levulinic acid | Clostridium acetobutylicum |
6 | - |
with acetoacetate | Clostridium acetobutylicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | activity range, profile overview | Clostridium acetobutylicum |