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Literature summary for 4.1.1.4 extracted from
- Acetoacetate decarboxylase (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 6, 255-270.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| rather easily inactivated by urea, guanidinium chloride and SDS | Clostridium acetobutylicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxopropane sulfonate | - |
Clostridium acetobutylicum |  |
| Acetopyruvate | - |
Clostridium acetobutylicum | |
| Acetylacetone | potent, reversible | Clostridium acetobutylicum | |
| beta-Diketones | - |
Clostridium acetobutylicum | |
| Borohydride | - |
Clostridium acetobutylicum | |
| HCN- | - |
Clostridium acetobutylicum | |
| Monovalent anions | at pH 7.0 and above, monovalent anions are entirely noncompetitive inhibitors, as the pH is lowered the inhibition becomes increasingly uncompetitive | Clostridium acetobutylicum | |
| p-chloromercuriphenyl sulfonate | - |
Clostridium acetobutylicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
acetoacetate | - |
Clostridium acetobutylicum | |
| 10 | - |
3-oxo-3-phenylpropionic acid | - |
Clostridium acetobutylicum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium acetobutylicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-oxo-3-phenylpropionic acid + H+ | - |
Clostridium acetobutylicum | ? | - |
? | |
| Acetoacetate | - |
Clostridium acetobutylicum | Acetone + CO2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
acetylacetone protects against thermal inactivation | Clostridium acetobutylicum |
| 25 | - |
pH 4-9, stable | Clostridium acetobutylicum |
| 70 | - |
above, biphasic irreversible inactivation | Clostridium acetobutylicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | 25°C, stable | Clostridium acetobutylicum |
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