Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Synechococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
F15L | the mutant shows reduced carboxylation activity compared to the wild type | Synechococcus sp. |
L138V | the mutant shows strongly reduced carboxylation activity compared to the wild type | Synechococcus sp. |
L27I | the mutant shows about wild type carboxylation activity | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | Synechococcus sp. | - |
3-phospho-D-glycerate + H+ | - |
? | |
D-ribulose 1,5-bisphosphate + CO2 + H2O | Synechococcus sp. OH28 | - |
3-phospho-D-glycerate + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | - |
- |
- |
Synechococcus sp. OH28 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
FPLC 15 Q column chromatography and Superose 12 gel filtration | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Synechococcus sp. | 3-phospho-D-glycerate + H+ | - |
? | |
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Synechococcus sp. OH28 | 3-phospho-D-glycerate + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexadecamer | - |
Synechococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
ribulose-1, 5-bisphosphate carboxylase/oxygenase | - |
Synechococcus sp. |
Rubisco | - |
Synechococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
7 | 9.5 | the melting temperature is around 79.5°C | Synechococcus sp. |