Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the S and L subunits of Rubisco are linked together using a flexible 40-amino acid tether. By replacing the rbcL in tobacco plastids with an artificial gene coding for a S40L fusion peptide, the fusions readily assemble into catalytic (S40L)8 and (S40L)16 oligomers that are devoid of unlinked S subunits. While there is little or no change in CO2/O2 specificity or carboxylation rate of the Rubisco oligomers, their Kms for CO2 and O2 are reduced 10% to 20% and 45%, respectively. In young maturing leaves of the plastome transformants (ANtS40L), the S40L-Rubisco levels are approximately 20% that of wild-type controls despite turnover of the S40L-Rubisco oligomers being only slightly enhanced relative to wild-type | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
CO2 | ANtS40L Rubisco oligomer complex, using soluble leaf protein extract | Nicotiana tabacum | |
0.0102 | - |
CO2 | rubisco oligomer (S40L)16 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification | Nicotiana tabacum | |
0.0106 | - |
CO2 | rubisco oligomer (S40L)8 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification | Nicotiana tabacum | |
0.0118 | - |
CO2 | wild-type, measured using fractions 12 and 13 following Superdex 200 HR purification | Nicotiana tabacum | |
0.0122 | - |
CO2 | wild-type, using soluble leaf protein extract | Nicotiana tabacum | |
0.112 | - |
O2 | ANtS40L Rubisco oligomer complex, using soluble leaf protein extract | Nicotiana tabacum | |
0.203 | - |
CO2 | wild-type, using soluble leaf protein extract | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Q-sepharose anion exchange chromatography and gel filtration | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Nicotiana tabacum | 2 3-phospho-D-glycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexadecamer | wild-type enzyme is an assembly of eight plastid-encoded large (L) and eight nucleus-encoded small (S) subunits froming a hexadecameric (L8S8) enzyme | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
Ribulose 1,5-bisphosphate carboxylase/oxygenase | - |
Nicotiana tabacum |
Rubisco | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
O2 | ANtS40L Rubisco oligomer complex, using soluble leaf protein extract | Nicotiana tabacum | |
0.65 | - |
CO2 | wild-type, using soluble leaf protein extract | Nicotiana tabacum | |
2.9 | - |
CO2 | ANtS40L Rubisco oligomer complex, using soluble leaf protein extract | Nicotiana tabacum | |
2.9 | - |
CO2 | rubisco oligomer (S40L)8 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification | Nicotiana tabacum | |
3.1 | - |
CO2 | rubisco oligomer (S40L)16 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification | Nicotiana tabacum | |
3.1 | - |
CO2 | wild-type, using soluble leaf protein extract | Nicotiana tabacum | |
3.3 | - |
CO2 | wild-type, measured using fractions 12 and 13 following Superdex 200 HR purification | Nicotiana tabacum |