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Literature summary for 4.1.1.39 extracted from

  • Ezaki, S.; Maeda, N.; Kishimoto, T.; Atomi, H.; Imanaka, T.
    Presence of a structurally novel type ribulose-bisphosphate carboxylase/oxygenase in the hyperthermophilic archaeon, Pyrococcus kodakaraensis KOD1 (1999), J. Biol. Chem., 274, 5078-5082.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49710
-
deduced from amino acid sequence Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 1,5-bisphosphate + CO2 + H2O Thermococcus kodakarensis first step in the fixation of athmospheric CO2 into the food chain 3-phospho-D-glycerate
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
KOD1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.8
-
-
Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 1,5-bisphosphate + CO2 + H2O
-
Thermococcus kodakarensis 3-phospho-D-glycerate
-
?
D-ribulose 1,5-bisphosphate + CO2 + H2O first step in the fixation of athmospheric CO2 into the food chain Thermococcus kodakarensis 3-phospho-D-glycerate
-
?
D-ribulose 1,5-bisphosphate + O2 competing side reaction Thermococcus kodakarensis 3-phospho-D-glycerate + 2-phosphoglycolate
-
?

Subunits

Subunits Comment Organism
homooctamer
-
Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
Pk-Rubisco
-
Thermococcus kodakarensis
ribulose-bisphosphate carboxylase/oxygenase
-
Thermococcus kodakarensis
Rubisco
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 100
-
Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80 100 extremely thermostable, half-life of 15 h at 80°C, 2.5 h at 100°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Thermococcus kodakarensis