BRENDA - Enzyme Database show
show all sequences of 4.1.1.38

Phosphoenolpyruvate carboxytransphosphorylase. II. Crystallization and properties

Lochmueller, H.; Wood, H.G.; Davis, J.J.; J. Biol. Chem. 241, 5678-5691 (1966)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Propionibacterium freudenreichii subsp. shermanii
General Stability
General Stability
Organism
0.05 M phosphate buffer, 0.01 M or 0.001 M thiol, 0.01 mg protein per ml, stable
Propionibacterium freudenreichii subsp. shermanii
30 h dialysis against phosphate buffer containing 1 mM mercaptoethanol, with a change of buffer at 15 h, stable
Propionibacterium freudenreichii subsp. shermanii
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Propionibacterium freudenreichii subsp. shermanii
diphosphate
at 1 mM
Propionibacterium freudenreichii subsp. shermanii
DL-malate
-
Propionibacterium freudenreichii subsp. shermanii
EDTA
-
Propionibacterium freudenreichii subsp. shermanii
glycylglycine
higher inhibition at pH 7.8 than at pH 6.5
Propionibacterium freudenreichii subsp. shermanii
KCl
at pH 6.5
Propionibacterium freudenreichii subsp. shermanii
MgCl2
at 7 mM
Propionibacterium freudenreichii subsp. shermanii
NaCl
at pH 6.5
Propionibacterium freudenreichii subsp. shermanii
oxaloacetate
at 7 mM
Propionibacterium freudenreichii subsp. shermanii
PCMB
-
Propionibacterium freudenreichii subsp. shermanii
SO42-
competitive against phosphate
Propionibacterium freudenreichii subsp. shermanii
Tris-HCl
higher inhibition at pH 7.8 than at pH 6.5
Propionibacterium freudenreichii subsp. shermanii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
can partially replace Mn2+ or Mg2+ in activation
Propionibacterium freudenreichii subsp. shermanii
Mg2+
Mn2+ or Mg2+ required
Propionibacterium freudenreichii subsp. shermanii
Mn2+
Mn2+ or Mg2+ required
Propionibacterium freudenreichii subsp. shermanii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
430000
-
low speed sedimentation without reaching equilibrium
Propionibacterium freudenreichii subsp. shermanii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Propionibacterium freudenreichii subsp. shermanii
-
-
-
Purification (Commentary)
Commentary
Organism
-
Propionibacterium freudenreichii subsp. shermanii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Propionibacterium freudenreichii subsp. shermanii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phosphate + phosphoenolpyruvate + CO2
-
33091
Propionibacterium freudenreichii subsp. shermanii
diphosphate + oxaloacetate
-
33091
Propionibacterium freudenreichii subsp. shermanii
r
phosphoenolpyruvate + phosphate
reaction is catalyzed in absence of CO2
33091
Propionibacterium freudenreichii subsp. shermanii
pyruvate + diphosphate
-
33091
Propionibacterium freudenreichii subsp. shermanii
ir
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1
8
formation of oxaloacetate
Propionibacterium freudenreichii subsp. shermanii
7
7.5
formation of phosphoenolpyruvate from oxaloacetate and diphosphate
Propionibacterium freudenreichii subsp. shermanii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.5
pH 5.5: about 35% of maximal activity, pH 8.5: about 55% of maximal activity, formation of oxalacetate
Propionibacterium freudenreichii subsp. shermanii
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
inactivation, even at 0°C
Propionibacterium freudenreichii subsp. shermanii
4.5
-
25°C, 5 h, little loss of activity
Propionibacterium freudenreichii subsp. shermanii
8.2
-
25°C, 0.2 M NaHCO3, 60% loss of activity after 30 min, rapid loss of activity above pH 8.2
Propionibacterium freudenreichii subsp. shermanii
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Propionibacterium freudenreichii subsp. shermanii
General Stability (protein specific)
General Stability
Organism
0.05 M phosphate buffer, 0.01 M or 0.001 M thiol, 0.01 mg protein per ml, stable
Propionibacterium freudenreichii subsp. shermanii
30 h dialysis against phosphate buffer containing 1 mM mercaptoethanol, with a change of buffer at 15 h, stable
Propionibacterium freudenreichii subsp. shermanii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Propionibacterium freudenreichii subsp. shermanii
diphosphate
at 1 mM
Propionibacterium freudenreichii subsp. shermanii
DL-malate
-
Propionibacterium freudenreichii subsp. shermanii
EDTA
-
Propionibacterium freudenreichii subsp. shermanii
glycylglycine
higher inhibition at pH 7.8 than at pH 6.5
Propionibacterium freudenreichii subsp. shermanii
KCl
at pH 6.5
Propionibacterium freudenreichii subsp. shermanii
MgCl2
at 7 mM
Propionibacterium freudenreichii subsp. shermanii
NaCl
at pH 6.5
Propionibacterium freudenreichii subsp. shermanii
oxaloacetate
at 7 mM
Propionibacterium freudenreichii subsp. shermanii
PCMB
-
Propionibacterium freudenreichii subsp. shermanii
SO42-
competitive against phosphate
Propionibacterium freudenreichii subsp. shermanii
Tris-HCl
higher inhibition at pH 7.8 than at pH 6.5
Propionibacterium freudenreichii subsp. shermanii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
can partially replace Mn2+ or Mg2+ in activation
Propionibacterium freudenreichii subsp. shermanii
Mg2+
Mn2+ or Mg2+ required
Propionibacterium freudenreichii subsp. shermanii
Mn2+
Mn2+ or Mg2+ required
Propionibacterium freudenreichii subsp. shermanii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
430000
-
low speed sedimentation without reaching equilibrium
Propionibacterium freudenreichii subsp. shermanii
Purification (Commentary) (protein specific)
Commentary
Organism
-
Propionibacterium freudenreichii subsp. shermanii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Propionibacterium freudenreichii subsp. shermanii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phosphate + phosphoenolpyruvate + CO2
-
33091
Propionibacterium freudenreichii subsp. shermanii
diphosphate + oxaloacetate
-
33091
Propionibacterium freudenreichii subsp. shermanii
r
phosphoenolpyruvate + phosphate
reaction is catalyzed in absence of CO2
33091
Propionibacterium freudenreichii subsp. shermanii
pyruvate + diphosphate
-
33091
Propionibacterium freudenreichii subsp. shermanii
ir
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1
8
formation of oxaloacetate
Propionibacterium freudenreichii subsp. shermanii
7
7.5
formation of phosphoenolpyruvate from oxaloacetate and diphosphate
Propionibacterium freudenreichii subsp. shermanii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.5
pH 5.5: about 35% of maximal activity, pH 8.5: about 55% of maximal activity, formation of oxalacetate
Propionibacterium freudenreichii subsp. shermanii
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
inactivation, even at 0°C
Propionibacterium freudenreichii subsp. shermanii
4.5
-
25°C, 5 h, little loss of activity
Propionibacterium freudenreichii subsp. shermanii
8.2
-
25°C, 0.2 M NaHCO3, 60% loss of activity after 30 min, rapid loss of activity above pH 8.2
Propionibacterium freudenreichii subsp. shermanii
Other publictions for EC 4.1.1.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748197
Chiba
Discovery of PPi-type phospho ...
Entamoeba histolytica
J. Biol. Chem.
290
23960-23970
2015
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1
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1
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2
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3
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1
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3
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3
2
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3
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3
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6
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3
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3
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3
6
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726738
Zhou
Atypical glycolysis in Clostri ...
no activity in Clostridium thermocellum
Appl. Environ. Microbiol.
79
3000-3008
2013
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1
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662039
Liu
Phosphoenolpyruvate carboxykin ...
Agrobacterium tumefaciens
J. Bacteriol.
187
6039-6045
2005
-
-
-
-
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1
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1
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2
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660748
Kim
Effect of overexpression of Ac ...
Actinobacillus succinogenes
Appl. Environ. Microbiol.
70
1238-1241
2004
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1
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1
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641615
Asanuma
Molecular characterization, en ...
Selenomonas ruminantium
Microbiology
147
681-690
2001
1
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2
-
2
1
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1
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1
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1
1
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1
1
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1
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2
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2
1
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1
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1
1
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1
1
-
-
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33096
Wood
Phosphorylation enzymes of the ...
Acidipropionibacterium acidipropionici, Propionibacterium freudenreichii, Propionibacterium freudenreichii subsp. shermanii
Proc. Natl. Acad. Sci. USA
82
312-315
1985
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3
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3
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3
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3
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3
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3
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33097
Eden
-
Autotrophic CO2 fixation in Ac ...
Acetobacterium woodii
Arch. Microbiol.
135
68-73
1983
1
-
-
-
-
-
-
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1
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1
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1
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1
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2
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33093
Perl
Phosphoenol-pyruvate-carboxyla ...
Gossypium hirsutum, Sorghum bicolor
Planta
139
239-243
1978
1
-
-
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2
3
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2
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2
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2
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4
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2
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2
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4
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2
2
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33098
Wood
Properties of carboxytransphos ...
Propionibacterium freudenreichii subsp. shermanii
Adv. Enzymol. Relat. Areas Mol. Biol.
45
85-155
1977
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6
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3
3
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6
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3
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2
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33094
O'Brien
Carboxytransphosphorylase. 8. ...
Propionibacterium freudenreichii subsp. shermanii
J. Biol. Chem.
249
4917-4925
1974
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1
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1
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4
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33092
Willard
Formation of enolpyruvate in t ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
12
5241-5246
1973
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2
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33095
O'Brien
Phosphoenolpyruvate carboxytra ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
12
5247-5253
1973
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33091
Lochmueller
Phosphoenolpyruvate carboxytra ...
Propionibacterium freudenreichii subsp. shermanii
J. Biol. Chem.
241
5678-5691
1966
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1
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12
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1
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1
3
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1
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12
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1
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1
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