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Literature summary for 4.1.1.37 extracted from

  • Nagaraj, V.A.; Arumugam, R.; Chandra, N.R.; Prasad, D.; Rangarajan, P.N.; Padmanaban, G.
    Localisation of Plasmodium falciparum uroporphyrinogen III decarboxylase of the heme-biosynthetic pathway in the apicoplast and characterisation of its catalytic properties (2008), Int. J. Parasitol., 39, 559-568.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol absolute requirement for activity Plasmodium falciparum
dithiothreitol absolute requirement for activity Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
native enzyme and N-terminally truncated UROD are expressed in Escherichia coli Rosetta2(DE3)pLysS cells Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
K150T the mutation does not have a significant effect on Vmax, but decreases Km by 25% Plasmodium falciparum
K150T/K219E the Km of the double mutant decreases by around 40% Plasmodium falciparum
K150T/K219E/K277P the mutant shows no significant change in Km, but the Vmax increases by 3.7fold Plasmodium falciparum
K277P the mutant decreases Km by 40% Plasmodium falciparum

General Stability

General Stability Organism
recombinant enzymes are catalytically less efficient compared with the host enzyme Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00072
-
uroporphyrinogen III native enzyme Homo sapiens
0.0087
-
uroporphyrinogen III recombinant enzyme Plasmodium falciparum
0.0109
-
Uroporphyrinogen I recombinant enzyme Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
apicoplast
-
Plasmodium falciparum 20011
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
SDS-PAGE Homo sapiens
43000
-
SDS-PAGE Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens
Ni-NTA column chromatography Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C or 4°C, 50 mM sodium phosphate buffer, pH 7.5, containing 300 mM imidazole, 1% Triton X-100, 50 mM NaCl, and 10% glycerol, more than a week, significant loss in activity Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uroporphyrinogen I
-
Homo sapiens coproporphyrinogen I + 4 CO2
-
?
uroporphyrinogen I
-
Plasmodium falciparum coproporphyrinogen I + 4 CO2
-
?
Uroporphyrinogen III
-
Homo sapiens Coproporphyrinogen III + 4 CO2
-
?
Uroporphyrinogen III
-
Plasmodium falciparum Coproporphyrinogen III + 4 CO2
-
?

Subunits

Subunits Comment Organism
dimer Superdex-G200 gel filtration Homo sapiens
dimer Superdex-G200 gel filtration Plasmodium falciparum

Synonyms

Synonyms Comment Organism
UroD
-
Homo sapiens
UroD
-
Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
recombinant enzyme Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
pH optimum around 7, recombinant enzyme Plasmodium falciparum

pI Value

Organism Comment pI Value Maximum pI Value
Plasmodium falciparum calculated from amino acid sequence
-
8.4