Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | absolute requirement for activity | Plasmodium falciparum | |
dithiothreitol | absolute requirement for activity | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
native enzyme and N-terminally truncated UROD are expressed in Escherichia coli Rosetta2(DE3)pLysS cells | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
K150T | the mutation does not have a significant effect on Vmax, but decreases Km by 25% | Plasmodium falciparum |
K150T/K219E | the Km of the double mutant decreases by around 40% | Plasmodium falciparum |
K150T/K219E/K277P | the mutant shows no significant change in Km, but the Vmax increases by 3.7fold | Plasmodium falciparum |
K277P | the mutant decreases Km by 40% | Plasmodium falciparum |
General Stability | Organism |
---|---|
recombinant enzymes are catalytically less efficient compared with the host enzyme | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00072 | - |
uroporphyrinogen III | native enzyme | Homo sapiens | |
0.0087 | - |
uroporphyrinogen III | recombinant enzyme | Plasmodium falciparum | |
0.0109 | - |
Uroporphyrinogen I | recombinant enzyme | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apicoplast | - |
Plasmodium falciparum | 20011 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
SDS-PAGE | Homo sapiens |
43000 | - |
SDS-PAGE | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Ni-NTA column chromatography | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C or 4°C, 50 mM sodium phosphate buffer, pH 7.5, containing 300 mM imidazole, 1% Triton X-100, 50 mM NaCl, and 10% glycerol, more than a week, significant loss in activity | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uroporphyrinogen I | - |
Homo sapiens | coproporphyrinogen I + 4 CO2 | - |
? | |
uroporphyrinogen I | - |
Plasmodium falciparum | coproporphyrinogen I + 4 CO2 | - |
? | |
Uroporphyrinogen III | - |
Homo sapiens | Coproporphyrinogen III + 4 CO2 | - |
? | |
Uroporphyrinogen III | - |
Plasmodium falciparum | Coproporphyrinogen III + 4 CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | Superdex-G200 gel filtration | Homo sapiens |
dimer | Superdex-G200 gel filtration | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
UroD | - |
Homo sapiens |
UroD | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
pH optimum around 7, recombinant enzyme | Plasmodium falciparum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plasmodium falciparum | calculated from amino acid sequence | - |
8.4 |