KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
uroporphyrinogen III | - |
Parachlorella kessleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE, monomer | Parachlorella kessleri |
90000 | - |
SDS-PAGE, active dimeric form | Parachlorella kessleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parachlorella kessleri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, Superdex 75 gel filtration | Parachlorella kessleri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
uroporphyrinogen III = coproporphyrinogen III + 4 CO2 | the decarboxylation of uroporphyrinogen occurs in two stages, via 7 COOH and then 6 and 5 COOH intermediates, with the decarboxylation of the 7 COOH compound being the rate-limiting step for the reaction | Parachlorella kessleri |
Storage Stability | Organism |
---|---|
-20°C, in 30-70% ammonium saturation fraction, 2 weeks, remains stable | Parachlorella kessleri |
-20°C, in 30-70% ammonium saturation fraction, 8 weeks, 50% loss of activity | Parachlorella kessleri |
-20°C, unprecipitated protein, 2 weeks, 36% loss of activity | Parachlorella kessleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Uroporphyrinogen III | - |
Parachlorella kessleri | Coproporphyrinogen III + 4 CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Parachlorella kessleri |
Synonyms | Comment | Organism |
---|---|---|
UroD | - |
Parachlorella kessleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
activity increases up to 45°C | Parachlorella kessleri |
65 | - |
activity decreases sharply at 65°C | Parachlorella kessleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Parachlorella kessleri |