Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.37 extracted from

  • Juarez, A.B.; Aldonatti, C.; Vigna, M.S.; Rios de Molina, M.d.e.l.C.
    Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta) (2007), Can. J. Microbiol., 53, 303-312.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
uroporphyrinogen III
-
Parachlorella kessleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE, monomer Parachlorella kessleri
90000
-
SDS-PAGE, active dimeric form Parachlorella kessleri

Organism

Organism UniProt Comment Textmining
Parachlorella kessleri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, Superdex 75 gel filtration Parachlorella kessleri

Reaction

Reaction Comment Organism Reaction ID
uroporphyrinogen III = coproporphyrinogen III + 4 CO2 the decarboxylation of uroporphyrinogen occurs in two stages, via 7 COOH and then 6 and 5 COOH intermediates, with the decarboxylation of the 7 COOH compound being the rate-limiting step for the reaction Parachlorella kessleri

Storage Stability

Storage Stability Organism
-20°C, in 30-70% ammonium saturation fraction, 2 weeks, remains stable Parachlorella kessleri
-20°C, in 30-70% ammonium saturation fraction, 8 weeks, 50% loss of activity Parachlorella kessleri
-20°C, unprecipitated protein, 2 weeks, 36% loss of activity Parachlorella kessleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Uroporphyrinogen III
-
Parachlorella kessleri Coproporphyrinogen III + 4 CO2
-
?

Subunits

Subunits Comment Organism
dimer gel filtration Parachlorella kessleri

Synonyms

Synonyms Comment Organism
UroD
-
Parachlorella kessleri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
activity increases up to 45°C Parachlorella kessleri
65
-
activity decreases sharply at 65°C Parachlorella kessleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Parachlorella kessleri