Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.37 extracted from

  • Jones, R.M.; Jordan, P.M.
    Purification and properties of the uroporphyrinogen decarboxylase from Rhodobacter sphaeroides (1993), Biochem. J., 293, 703-712.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates Cereibacter sphaeroides

General Stability

General Stability Organism
unstable in absence of 2-mercaptoethanol Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Cereibacter sphaeroides
Coproporphyrin I
-
Cereibacter sphaeroides
coproporphyrin III
-
Cereibacter sphaeroides
Cu2+
-
Cereibacter sphaeroides
Fe3+
-
Cereibacter sphaeroides
Haematoporphyrin IX
-
Cereibacter sphaeroides
Haemin
-
Cereibacter sphaeroides
Hg2+
-
Cereibacter sphaeroides
iodoacetamide
-
Cereibacter sphaeroides
iodoacetate
-
Cereibacter sphaeroides
mesoporphyrin IX
-
Cereibacter sphaeroides
NEM
-
Cereibacter sphaeroides
Phenylglyoxal
-
Cereibacter sphaeroides
protoporphyrin IX
-
Cereibacter sphaeroides
uroporphyrin III
-
Cereibacter sphaeroides
Uroporphyrinogen I
-
Cereibacter sphaeroides
uroporphyrinogen III
-
Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
Uroporphyrinogen I
-
Cereibacter sphaeroides
0.006
-
uroporphyrinogen III
-
Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ slight activation Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000 39000 gel filtration, nondenaturing PAGE Cereibacter sphaeroides
41000
-
1 * 41000, SDS-PAGE Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0126
-
-
Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
frozen in 25 mM potassium phosphate buffer, pH 7.0, containing 2-mercaptoethanol, stable Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uroporphyrinogen I
-
Cereibacter sphaeroides coproporphyrinogen I + 4 CO2
-
?
Uroporphyrinogen III
-
Cereibacter sphaeroides Coproporphyrinogen III + 4 CO2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
t1/2 = 8 min Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 6.9
-
Cereibacter sphaeroides
6.8
-
-
Cereibacter sphaeroides

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.7 pH 6.2: about 25% of maximal activity, pH 7.7: about 70% of maximal activity Cereibacter sphaeroides

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
unstable below Cereibacter sphaeroides