Crystallization (Comment) | Organism |
---|---|
purified enzyme in complex with (R)-5-diphosphomevalonate and adenosine 5'-O-(3-thio)triphosphate or with (R)-5-diphosphomevalonate and ADP, X-ray diffraction structure determination and analysis at 1.5-1.7 A resolution | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D281N | site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity and phosphate elimination/decarboxylation | Saccharolobus solfataricus |
D281T | site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation | Saccharolobus solfataricus |
D281V | site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation | Saccharolobus solfataricus |
additional information | the DMD activity, which yields IPP from MVA-5-PP in the presence of ATP, is observed only with the wild type and with the D281N mutant | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-diphosphomevalonate | Saccharolobus solfataricus | - |
ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | Saccharolobus solfataricus P2 | - |
ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | Saccharolobus solfataricus JCM 11322 | - |
ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | Saccharolobus solfataricus ATCC 35092 | - |
ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | Saccharolobus solfataricus DSM 1617 | - |
ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97UL5 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus ATCC 35092 | Q97UL5 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus DSM 1617 | Q97UL5 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus JCM 11322 | Q97UL5 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus P2 | Q97UL5 | i.e. Sulfolobus solfataricus | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO2 | catalytic mechanism of this rare type of decarboxylase, the enzyme is bifunctional showing the reactions of diphosphomevalonate decarboxylase (EC 4.1.1.33) and mevalonate 3-kinase (EC 2.7.1.185), overview | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-diphosphomevalonate | - |
Saccharolobus solfataricus | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | formation of isopentenyl diphosphate through the ATP-dependent phosphorylation of the 3-hydroxyl group of (R)-5-diphosphomevalonate followed by decarboxylation coupled with the elimination of the 3-phosphate group | Saccharolobus solfataricus | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | - |
Saccharolobus solfataricus P2 | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | formation of isopentenyl diphosphate through the ATP-dependent phosphorylation of the 3-hydroxyl group of (R)-5-diphosphomevalonate followed by decarboxylation coupled with the elimination of the 3-phosphate group | Saccharolobus solfataricus P2 | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | - |
Saccharolobus solfataricus JCM 11322 | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | formation of isopentenyl diphosphate through the ATP-dependent phosphorylation of the 3-hydroxyl group of (R)-5-diphosphomevalonate followed by decarboxylation coupled with the elimination of the 3-phosphate group | Saccharolobus solfataricus JCM 11322 | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | - |
Saccharolobus solfataricus ATCC 35092 | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | formation of isopentenyl diphosphate through the ATP-dependent phosphorylation of the 3-hydroxyl group of (R)-5-diphosphomevalonate followed by decarboxylation coupled with the elimination of the 3-phosphate group | Saccharolobus solfataricus ATCC 35092 | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | - |
Saccharolobus solfataricus DSM 1617 | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + (R)-5-diphosphomevalonate | formation of isopentenyl diphosphate through the ATP-dependent phosphorylation of the 3-hydroxyl group of (R)-5-diphosphomevalonate followed by decarboxylation coupled with the elimination of the 3-phosphate group | Saccharolobus solfataricus DSM 1617 | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
additional information | NMR analysis of the product | Saccharolobus solfataricus | ? | - |
- |
|
additional information | NMR analysis of the product | Saccharolobus solfataricus P2 | ? | - |
- |
|
additional information | NMR analysis of the product | Saccharolobus solfataricus JCM 11322 | ? | - |
- |
|
additional information | NMR analysis of the product | Saccharolobus solfataricus ATCC 35092 | ? | - |
- |
|
additional information | NMR analysis of the product | Saccharolobus solfataricus DSM 1617 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
DMD | - |
Saccharolobus solfataricus |
More | see also EC 2.7.1.185 | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
malfunction | site-directed mutagenesis on Asp281 creates mutants that only show diphosphomevalonate 3-kinase activity, demonstrating that the residue is required in the process of phosphate elimination/decarboxylation, rather than in the preceding phosphorylation step | Saccharolobus solfataricus |
additional information | the conserved aspartate residue, Asp281, shows inability for proton abstraction. Substrate-complex structures of DMD and M3K (EC 2.7.1.185), overview | Saccharolobus solfataricus |
physiological function | the biosynthesis of isopentenyl diphosphate, a fundamental precursor for isoprenoids, via the mevalonate pathway is completed by diphosphomevalonate decarboxylase. This enzyme catalyzes the formation of isopentenyl diphosphate through the ATP-dependent phosphorylation of the 3-hydroxyl group of (R)-5-diphosphomevalonate followed by decarboxylation coupled with the elimination of the 3-phosphate group. Involvement of a long predicted intermediate, (R)-3-phospho-5-diphosphomevalonate, in the reaction of the enzyme | Saccharolobus solfataricus |