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Literature summary for 4.1.1.33 extracted from

  • Hattori, A.; Unno, H.; Goda, S.; Motoyama, K.; Yoshimura, T.; Hemmi, H.
    In vivo formation of the protein disulfide bond that enhances the thermostability of diphosphomevalonate decarboxylase, an intracellular enzyme from the hyperthermophilic archaeon Sulfolobus solfataricus (2015), J. Bacteriol., 197, 3463-3471.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.33

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
C210S decrease in melting temperature by 12 degrees Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, calculated and dynamic light scattering Saccharolobus solfataricus
42000
-
 gel filtration, Tris-HCl buffer, presence of 2-mercaptoethanol Saccharolobus solfataricus
52000
-
 gel filtration, sodium phosphate buffer Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97UL5
-
-
Saccharolobus solfataricus DSM 1617 Q97UL5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification subunits of the enzyme homodimer are connected via an intersubunit disulfide bond between the Cys210 residues. When mutagenesis replaces the disulfide-forming cysteine residue with serine, the thermostability of the enzyme is significantly lowered. In the presence of 2-mercaptoethanol, the wild-type enzyme is less stable to heat. The disulfide bond is predominantly formed in the cells Saccharolobus solfataricus

Subunits

Subunits Comment Organism
dimer 2 * 37000, calculated and dynamic light scattering Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SSO2989
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
78
-
 melting temperature, mutant C210S Saccharolobus solfataricus
80
-
 1 h, complete loss of activity in presence of 2-mercaptoethanol Saccharolobus solfataricus
90
-
 melting temperature, wild-type Saccharolobus solfataricus