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Literature summary for 4.1.1.33 extracted from
- Formation of isobutene from 3-hydroxy-3-methylbutyrate by diphosphomevalonate decarboxylase (2010), Appl. Environ. Microbiol., 76, 85004-8010.
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | enzyme establishes a possible route for biological production of petroleum based fuels and plastics by producing isobutene enzymatically | Saccharomyces cerevisiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells. Whole cells of Eschercihia coli produced ScMDD with an N-terminal 6xHis tag form isobutene from 3-hydroxy-3-methylbutyrate at a rate 0.154 nmol/h * g cells | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R74H | mutant increases specific activity on non-native substrate (3-hydroxy-3-methylbutyrate) by 4.8fold | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
| 44120 | - |
calculated from protein sequence | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-diphosphomevalonate | Saccharomyces cerevisiae | - |
ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P32377 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both, the wild-type 6XHis tag ScMDD and the most active mutant R74H ScMDD2 purified by nickel affinity chromatography under anaerobic conditions, 90 to 95% homogenous | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
improved mutants ScMDD1 (I145F) and ScMDD2 (R74H) produce 3 and 5.888 nmol/h * g cells, respectively, which are 19- and 38fold increases compared to the wild type 6xHis ScMDD | Saccharomyces cerevisiae |
| 0.00000133 | - |
wild-type, 6xHis tagged, produced in Escherichia coli, 37°C, pH 7.4 | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-hydroxy-3-methylbutyrate | - |
Saccharomyces cerevisiae | ADP + isobutene + CO2 | isobutene is identified by gas chromatography with flame ionization detection as well as by GC-mass spectrometry. Isobutene is an important commercial chemical used for the synthesis of butyl rubber, terephthalic acid, specialty chemicals, and a gasoline performance additive known as alkylate | ? | |
| ATP + 5-diphosphomevalonate | - |
Saccharomyces cerevisiae | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diphosphomevalonate decarboxylase | - |
Saccharomyces cerevisiae |
| ScMDD | - |
Saccharomyces cerevisiae |
| ScMDD1 | improved mutant I145F | Saccharomyces cerevisiae |
| ScMDD2 | improved mutant R74H | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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