Literature summary for 4.1.1.33 extracted from

Voynova, N.E.; Fu, Z.; Battaile, K.P.; Herdendorf, T.J.; Kim, J.J.; Miziorko, H.M.
Human mevalonate diphosphate decarboxylase: characterization, investigation of the mevalonate diphosphate binding site, and crystal structure (2008), Arch. Biochem. Biophys., 480, 58-67.

Search for more literature for 4.1.1.33

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapour diffusion method, with 0.1 M MES buffer pH 6.5, 20% PEG 5000 MME, 0.1 M NaCl, 1 mM dithiothreitol, and 0.2 M ammonium sulfate	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
N17A	mutant exhibits a 15fold inflation in Km for 5-diphosphomevalonate	Homo sapiens
R161Q	mutant exhibits an about 1000fold diminution in specific activity, while binding the fluorescent substrate analog, TNP-ATP, comparably to wild type enzyme	Homo sapiens
S162A	mutant shows reduced activity with 2fold decrease in Vm and 4 to 7fold increases in substrate Km values	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
6-fluoromevalonate 5-diphosphate	competitive inhibitor with respect to 5-diphosphomevalonate	Homo sapiens
diphosphoglycolyl proline	competitive inhibitor with respect to 5-diphosphomevalonate	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0289	-	5-diphosphomevalonate	wild type enzyme, at 30°C	Homo sapiens
0.1257	-	5-diphosphomevalonate	mutant enzyme S162A, at 30°C	Homo sapiens
0.425	-	5-diphosphomevalonate	mutant enzyme N17A, at 30°C	Homo sapiens
0.69	-	ATP	wild type enzyme, at 30°C	Homo sapiens
4.9	-	ATP	mutant enzyme S162A, at 30°C	Homo sapiens
7.7	-	ATP	mutant enzyme N17A, at 30°C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	20 mM, required for activity	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P53602	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-Sepharose resin chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 5-diphosphomevalonate	-	Homo sapiens	ADP + phosphate + isopentenyl diphosphate + CO2	-	?

Subunits

Subunits	Comment	Organism
dimer	x-ray crystallography	Homo sapiens

Synonyms

Synonyms	Comment	Organism
MDD	-	Homo sapiens
mevalonate diphosphate decarboxylase	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0022	-	5-diphosphomevalonate	mutant enzyme R161Q, at 30°C	Homo sapiens
0.2	-	5-diphosphomevalonate	mutant enzyme N17A, at 30°C	Homo sapiens
2.1	-	5-diphosphomevalonate	mutant enzyme S162A, at 30°C	Homo sapiens
4.5	-	5-diphosphomevalonate	wild type enzyme, at 30°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000062	-	6-fluoromevalonate 5-diphosphate	wild type enzyme	Homo sapiens
0.0023	-	diphosphoglycolyl proline	wild type enzyme	Homo sapiens

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Release 2023.1 (January 2023)