Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Trypanosoma brucei |
expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
- |
Trypanosoma brucei |
hanging drop vapour diffusion method using 0.1 M Tris-HCl (pH 8.0), 1.8 M sodium malonate | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36800 | - |
SDS-PAGE | Trypanosoma brucei |
36800 | - |
SDS-PAGE | Staphylococcus aureus |
44100 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32377 | - |
- |
Staphylococcus aureus | A0A0H2XIV6 | - |
- |
Trypanosoma brucei | Q388P2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ chelating column chromatography and HiTrapQ Sepharose column chromatography | Trypanosoma brucei |
Ni2+ chelating column chromatography and HiTrapQ Sepharose column chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-diphosphomevalonate | - |
Saccharomyces cerevisiae | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + 5-diphosphomevalonate | - |
Trypanosoma brucei | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
ATP + 5-diphosphomevalonate | - |
Staphylococcus aureus | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | in solution, gel filtration | Staphylococcus aureus |
monomer | in solution, gel filtration | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
MDD | - |
Saccharomyces cerevisiae |
MDD | - |
Trypanosoma brucei |
MDD | - |
Staphylococcus aureus |
mevalonate diphosphate decarboxylase | - |
Saccharomyces cerevisiae |
mevalonate diphosphate decarboxylase | - |
Trypanosoma brucei |
mevalonate diphosphate decarboxylase | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae | |
ATP | - |
Trypanosoma brucei | |
ATP | - |
Staphylococcus aureus |