Any feedback?
Literature summary for 4.1.1.32 extracted from
- Kinetic characterization of recombinant human cytosolic phosphoenolpyruvate carboxykinase with and without a His10-tag (2007), Biochim. Biophys. Acta, 1770, 1576-1584.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activity regulator, 0.7 mM leads to full enzyme stimulation | Homo sapiens |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | plays a role in the development of type 2 diabetes | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C41(DE3) cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KHCO3 | above 0.1 M inhibits the oxaloacetate-forming activity | Homo sapiens | |
| Mn2+ | Mn2+ concentrations above 0.7 mM inhibit the His-tagged enzyme | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
oxaloacetate | in 100 mM HEPES-NaOH, pH 7.2, 100 mM KHCO3, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C | Homo sapiens | |
| 0.023 | - |
GTP | in 100 mM HEPES-NaOH, pH 7.2, 100 mM KHCO3, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C | Homo sapiens | |
| 0.45 | - |
phosphoenolpyruvate | in 100 mM HEPES-NaOH, pH 8, 3.6 mM L-malate, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C | Homo sapiens | |
| 20.6 | - |
GDP | in 100 mM HEPES-NaOH, pH 8, 3.6 mM L-malate, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA Superflow agarose column chromatography and Ekapture-agarose column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + oxaloacetate | - |
Homo sapiens | GDP + phosphoenolpyruvate + CO2 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GTP-PEPCK | - |
Homo sapiens |
| phosphoenolpyruvate carboxykinase, GTP-dependent | - |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
- |
Homo sapiens | KHCO3 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
